1. Antibodies
  2. Homo sapiens
  3. USF2

ENCAB940OHP

Antibody against Homo sapiens USF2

Homo sapiens
K562
characterized to standards
Status
released
Source (vendor)
Abcam
Product ID
ab60931
Lot ID
GR40775
Characterized targets
USF2 (Homo sapiens)
Host
mouse
Clonality
monoclonal
Purification
Protein A
Isotype
IgG2a
Antigen description
Recombinant fragment with tag: MDMLDPGLDP AASATAAAAA SHDKGPEAEE GVELQEGGDG PGAEEQTAVA ITSVQQAAFG DHNIQYQFRT ETNGGQVTYR VVQVTDGQLD GQGDTAGAVS , corresponding to amino acids 1-101 of Human USF2
Aliases
michael-snyder:AS-61
External resources

Characterizations

USF2 (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
compliant
Caption
Immunoprecipitation of USF2 from K562 cells using ab60931. Lane 1: input nuclear lysate. Lane 2: material immunoprecipitated with ab60931. Lane 3: material immunoprecipitated using control IgG. Band A was excised from gel and subject to analysis by mass spectrometry. Expected band size ~37kD.
Reviewer comment
How come not all immunoreactive bands were excised for mass-spec analysis?
Submitted by
Kathrina Onate
Lab
Michael Snyder, Stanford
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 USF2_IP.png
USF2 (Homo sapiens)
Method: immunoprecipitation followed by mass spectrometry
Attachment PDF Icon
compliant
Caption
IP followed by mass spectrometry: Briefly, protein was immunoprecipitated from K562 whole cell lysates using ab60931, and the IP fraction was loaded on a 10% polyacrylamide gel (NuPAGE Bis-Tris Gel) and separated with an Invitrogen NuPAGE electrophoresis system. The gel was silver-stained, gel fragments corresponding to the bands indicated were excised and destained using the SilverSNAP Stain for Mass Spectrometry (Pierce). Then proteins were treated with trypsin using the in-gel digestion method. Digested proteins were analyzed on a LTQ-Orbitrap (Thermo Scientific) b the nanoLC-ESI-MS/MS technique. Peptides were identified by the SEQUEST algorithm and filtered with a high confidence threshold (protein false discovery rate <1%, 2 peptides per protein minimum).
Submitted by
Kathrina Onate
Lab
Michael Snyder, Stanford