1. Antibodies
  2. Homo sapiens
  3. LCORL

ENCAB917SDR

Antibody against Homo sapiens LCORL

Homo sapiens
HepG2
characterized to standards with exemption
Status
released
Source (vendor)
Sigma
Product ID
HPA028794
Lot ID
R28271
Characterized targets
LCORL (Homo sapiens)
Host
rabbit
Clonality
polyclonal
Purification
affinity
Isotype
IgG
Antigen description
ligand dependent nuclear receptor corepressor-like recombinant protein epitope signature tag (PrEST)
Antigen sequence
GPSIVCKSIQMNQAENSLQEEQEGPLDLTVNRMQEQNTQQGDGVLDLSTKKTSIKSEESSICDPSSENSVAGRLHRNREDYVERSAEFADGLL
Aliases
michael-snyder:752
External resources

Characterizations

LCORL (Homo sapiens)
HepG2
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: HepG2, using the antibody HPA028794. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.
Reviewer comment
Multiple bands but band at expected size just slightly >50% of the total signal in the lane.
Submitted by
Denis Salins
Lab
Michael Snyder, Stanford
LCORL (Homo sapiens)
Method: immunoprecipitation followed by mass spectrometry
Attachment PDF Icon
exempt from standards
Caption
IP followed by mass spectrometry. Briefly, protein was immunoprecipitated from HepG2 nuclear cell lysates using the antibody HPA028794, and the IP fraction was loaded on a 10% polyacrylamide gel (NuPAGEBis-Tris Gel) and separated with an Invitrogen NuPAGE electrophoresis system. The gel was stained by ColloidialCoomassie G-250 stain, gel fragments corresponding to the bands indicated were excised. Then proteins were trypsinized using the in-gel digestion method. Digested proteins were analyzed on an Orbitrap Elite mass spectrometer (Thermo Scientific) by the nanoLC-ESI-MS/MS technique. Peptides were identified by the SEQUEST algorithm and filtered with a high confidence threshold (Peptide false discovery rate < 1%, 2 unique peptides per protein minimum, mass error < 10 ppm).
Submitter comment
None of the proteins ranked above or equally ranked have been shown to be sequence-specific TFs (including LCOR).
Reviewer comment
Not all immunoreactive bands were analyzed but it's obviously too late to change that so we can only evaluate what was submitted. The targeted TF can be found in the excised region containing immunoreactive bands near the expected size.
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
LCORL (Homo sapiens)
HepG2
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
exempt from standards
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line HepG2 using the antibody HPA028794. Lane 1: input nuclear lysate. Lane 2: material immunoprecipitated with antibody. Lane 3: material immunoprecipitated using control IgG. Marked bands were excised from gel and subjected to analysis by mass spectrometry. Target molecular weight: 66.964.
Submitter comment
We show that the targeted TF is detected by mass-spec.
Reviewer comment
Multiple immunoreactive bands and marked band does not contain at least 50% of the total signal in the lane. However, the targeted TF was detected in the marked region by subsequent mass-spec analysis.
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford