ENCAB899PTZ

Antibody against Homo sapiens EGR1

Homo sapiens
MCF-7, K562
characterized to standards
Homo sapiens
any cell type or tissue
partially characterized
Homo sapiens
GM12878, HepG2
not characterized to standards
Status
released
Source (vendor)
Bethyl Labs
Product ID
A303-390A
Lot ID
1
Host
rabbit
Clonality
polyclonal
Purification
affinity
Aliases
michael-snyder:AS-1589
External resources

Characterizations

EGR1 (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: K562 using the antibody A303-390A. The image shows western blot analysis of input, flowthrough, immunoprecipitate, and mock immunoprecipitate using IgG. Target molecular weight: 57.507.
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
EGR1 (Homo sapiens)
Method: immunoprecipitation
Attachment from submitter
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: HEK293T, using the antibody A303-390A. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 57.507
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
EGR1 (Homo sapiens)
MCF-7
Method: immunoprecipitation
Attachment from submitter
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: MCF-7, using the antibody A303-390A. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 57.507
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
EGR1 (Homo sapiens)
GM12878
Method: immunoprecipitation
Attachment from submitter
not compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: GM12878, using the antibody A303-390A. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 57.507
Reviewer comment
KCO: Arrow pointing to 'expected band size' is actually not the expected band size; banding pattern between IP and IgG are very similar
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
EGR1 (Homo sapiens)
HepG2
Method: immunoprecipitation
Attachment from submitter
not compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: HepG2, using the antibody A303-390A. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 57.507
Submitter comment
<50% intensity
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
EGR1 (Homo sapiens)
Method: motif enrichment
compliant
Caption
The motif for target EGR1 is represented by the attached position weight matrix (PWM) derived from ENCFF004WYV. Motif enrichment analysis was done by Dr. Zhizhuo Zhang (Broad Institute, Kellis Lab) using known motifs (http://compbio.mit.edu/encode-motifs/) and previously published ChIP-seq data (http://www.broadinstitute.org/~zzhang/motifpipeline/data/TrainSetInfo.txt). The accept probability score of the given transcription factor was calculated using a Bayesian approach. This analysis also includes three motif enrichment scores, computed by overlapping the motif instances with the given ChIP-seq peak locations, as well as an enrichment rank. For more information on the underlying statistical methods, please see the attached document. Accept probability score: 0.836933619638 Global enrichment Z-score: 4.42731068816 Positional bias Z-score: 10.0090352815 Peak rank bias Z-score: 9.6649763368 4 Enrichment rank: 1.0
Submitted by
Jessika Adrian
Lab
Michael Snyder, Stanford