ENCAB874OIK
Antibody against Homo sapiens NFATC3
Homo sapiens
K562
partially characterized
Homo sapiens
HepG2
not characterized to standards
- Status
- released
- Source (vendor)
- GeneTex
- Product ID
- GTX52339
- Lot ID
- 821400567
- Characterized targets
- NFATC3 (Homo sapiens)
- Host
- rabbit
- Clonality
- polyclonal
- Purification
- affinity
- Isotype
- IgG
- Antigen description
- A synthesized peptide derived from human NFAT4 around the phosphorylation site of Ser165
- External resources
Characterizations
NFATC3 (Homo sapiens)
K562HepG2
Method: immunoprecipitation
compliant
- Caption
- Whole cell lysates of K562 and HepG2 were immunoprecipitated using the primary antibody (GeneTex; GTX52339). The IP fraction was separated on a 12% acrylamide gel with the Bio-Rad PROTEAN II xi system. After separation, the samples were transferred to a nitrocellulose membrane with an Invitrogen iBlot system. The membrane was probed with the primary antibody (same as that used for IP) and a secondary HRP-conjugated antibody. The resulting bands were visualized with SuperSignal West Femto Solution (Thermo Scientific). Protein Marker (PM) is labeled in kDa. Two bands were detected at ~80 and 103 kDa. Uniprot expected size is ~115 kDa
- Submitted by
- Mark Mackiewicz
- Lab
- Richard Myers, HAIB
- Grant
- U54HG006998
- Download
- NFATC3_Western.png