ENCAB872RJV

Homo sapiens

K562, GM12878

partially characterized

K562GM12878

Method: immunoblot

compliant

Caption: Nuclear lysates from K562 (10ug) and GM12878 (10ug) were loaded into a 4-12% Bis-Tris gel in 1X MESS running buffer. After separation, the samples were transferred to a nitrocellulose membrane using iblot. Membrane was blocked for an hour in room temperature, with 5% BSA in TBS-T and blotted with primary antibody in the appropriate concentration over night at 4c. Membrane was washed and blotted with secondary HRP-conjugated antibody. Detection was made with Optiblot ECL Detect Kit (ab133406) for 2 min. Bands were detected at the expected size (36kDa) and outside of the expected range as well. **Molecular mass: 61771 Da**
Submitted by: Noam Shoresh
Lab: Bradley Bernstein, Broad
Grant: U54HG006991
Download: BRCC3_BRCC36_Bethyl_A302-517A-1.png
Documents: encode:Antibody_Characterization_ENCODE3_August2015.pdf