ENCAB871WRG

Antibody against Homo sapiens POLR2A

Homo sapiens
breast epithelium, vagina, gastroesophageal sphincter, lower leg skin, thyroid gland, transverse colon, right lobe of liver, Peyer's patch, esophagus squamous epithelium, spleen, testis, subcutaneous adipose tissue, upper lobe of left lung, uterus, prostate gland, stomach, sigmoid colon, ovary, gastrocnemius medialis, tibial nerve, body of pancreas, esophagus muscularis mucosa, suprapubic skin, heart left ventricle, adrenal gland
characterized to standards with exemption
Status
released
Source (vendor)
BioLegend
Product ID
920102
Lot ID
B200433
Characterized targets
POLR2A (Homo sapiens)
Host
mouse
Clonality
monoclonal
Isotype
IgG2a
Aliases
michael-snyder:AS-1649
External resources

Characterizations

POLR2A (Homo sapiens)
Method: immunoprecipitation followed by mass spectrometry
Attachment from submitter
exempt from standards
Caption
Characterizations for this antibody were exempted for all cell types and tissues by the Antibody Review Panel on March 30, 2016.
Submitter comment
This is a well characterized antibody already used in many ENCODE experiments (clone 8WG16).
Reviewer comment
Characterizations for this antibody were exempted for all cell types and tissues by the Antibody Review Panel on March 30, 2016.
Submitted by
Esther Chan
Lab
Michael Snyder, Stanford
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POLR2A (Homo sapiens)
sigmoid colonstomachtransverse colonsubcutaneous adipose tissuegastroesophageal sphincterbreast epitheliumsuprapubic skinbody of pancreasuteruslower leg skinprostate glandupper lobe of left lungPeyer's patchovaryadrenal glandthyroid glandtestisesophagus squamous epitheliumvaginaheart left ventriclespleengastrocnemius medialisesophagus muscularis mucosaright lobe of livertibial nerve
Method: immunoblot
Attachment from submitter
exempt from standards
Caption
Primary characterization (e.g. immunoblot, immunoprecipitation) for the specified cell types and tissues are exempted for this antibody by the ENCODE antibody review panel because it is a well-characterized monoclonal antibody (clone 8WG16) that has been in use without issue by the community for many years.
Submitter comment
This is a well characterized antibody already used in many ENCODE experiments. It is also previously known as Convance cat# MMS-126R: http://www.biolegend.com/anti-rna-polymerase-ii-antibody-11145.html (clone 8WG16)
Reviewer comment
Primary characterizations for this antibody were exempted for all cell types and tissues by the Antibody Review Panel on March 30, 2016.
Submitted by
Esther Chan
Lab
Michael Snyder, Stanford
Download