ENCAB847KNU

Antibody against Homo sapiens EIF4A2

Homo sapiens
K562
partially characterized
Homo sapiens
at least one cell type or tissue
not pursued
Status
released
Source (vendor)
GeneTex
Product ID
GTX107484
Lot ID
40352
Characterized targets
EIF4A2 (Homo sapiens)
Host
rabbit
Clonality
polyclonal
Purification
affinity
Isotype
IgG
External resources

Characterizations

EIF4A2 (Homo sapiens)
Method: immunoprecipitation
Attachment from submitter
Caption
IP-WB characterization of EIF4A2 specific antibody in K562 cell line . Lane 1 is 2.5% of five million K562 whole cell lysate Input, lane 2 is 2.5% of supernatant after immunoprecipitation and Lane 3 is 50% of IP enrichment using rabbit polyclonal Anti-EIF4A2 antibody. This antibody passes preliminary validation and will be further pursued for primary and secondary validation.
Submitted by
Balaji Sundararaman
Lab
Gene Yeo, UCSD
EIF4A2 (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
compliant
Caption
IP-Western Blot analysis of K562 whole cell lysate using EIF4A2 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 10% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal Anti-EIF4A2 antibody (lanes under 'EIF4A2').
Submitted by
Balaji Sundararaman
Lab
Gene Yeo, UCSD