ENCAB846GVR
Antibody against Homo sapiens ATP5C1
Homo sapiens
K562
characterized to standards
Homo sapiens
any cell type or tissue
partially characterized
- Status
- released
- Source (vendor)
- GeneTex
- Product ID
- GTX114275
- Lot ID
- 40156
- Characterized targets
- ATP5C1 (Homo sapiens)
- Host
- rabbit
- Clonality
- polyclonal
- Purification
- affinity
- Isotype
- IgG
- External resources
Characterizations
ATP5C1 (Homo sapiens)
compliant
- Caption
- Western blot following shRNA against ATP5C1 in HepG2 whole cell lysate using ATP5C1 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against ATP5C1.ATP5C1 protein appears as the green band, Tubulin serves as a control and appears in red.
- Submitted by
- Xintao Wei
- Lab
- Brenton Graveley, UConn
- Grant
- U54HG007005
- Download
- ATP5C1-HEPG2_Secondary_Western.png
ATP5C1 (Homo sapiens)
compliant
- Caption
- Western blot following shRNA against ATP5C1 in K562 whole cell lysate using ATP5C1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against ATP5C1.ATP5C1 protein appears as the green band, Tubulin serves as a control and appears in red.
- Submitted by
- Xintao Wei
- Lab
- Brenton Graveley, UConn
- Grant
- U54HG007005
- Download
- ATP5C1-K562_Secondary_Western.png
ATP5C1 (Homo sapiens)
not submitted for review by lab
- Caption
- IP-WB analysis of K562 whole cell lysate using ATP5C1 specific antibody. Lane 1 is 2.5% of 0.5mg input lysate, lane 2 is 2.5% of supernatant after immunoprecipitation and Lane 3 is 50% of IP enrichment using rabbit polyclonal ATPsynthasegamma antibody. This antibody passes preliminary validation and will be further pursued for primary and secondary validation.
- Submitted by
- Balaji Sundararaman
- Lab
- Gene Yeo, UCSD
- Grant
- U54HG007005
- Download
- GeneTex_GTX114275_40156_ATP5C1.png
ATP5C1 (Homo sapiens)
K562
compliant
- Caption
- IP-Western Blot analysis of K562 whole cell lysate using ATP5C1 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 10% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal ATP synthase gamma antibody (lanes under 'ATP5C1').
- Submitted by
- Balaji Sundararaman
- Lab
- Gene Yeo, UCSD
- Grant
- U54HG007005