ENCAB835WNS

Antibody against Homo sapiens RCOR1

Homo sapiens
MCF-7, K562, A549, SK-N-SH
characterized to standards
Status
released
Source (vendor)
Santa Cruz Biotech
Product ID
sc-30189
Lot ID
C2511
Characterized targets
RCOR1 (Homo sapiens)
Host
rabbit
Clonality
polyclonal
Aliases
michael-snyder:AS-804
External resources

Characterizations

RCOR1 (Homo sapiens)
MCF-7
Method: immunoprecipitation
Attachment from submitter
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: MCF-7, using the antibody sc-30189. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.
Submitted by
Denis Salins
Lab
Michael Snyder, Stanford
RCOR1 (Homo sapiens)
A549
Method: immunoprecipitation
Attachment from submitter
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: A549 using the antibody sc-30189. The image shows western blot analysis of input, flowthrough, immunoprecipitate, and mock immunoprecipitate using IgG. Target molecular weight: 53.028.
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
RCOR1 (Homo sapiens)
SK-N-SH
Method: immunoprecipitation
Attachment from submitter
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: SK-N-SH using the antibody sc-30189. The image shows western blot analysis of input, flowthrough, immunoprecipitate, and mock immunoprecipitate using IgG. Target molecular weight: 53.028.
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
RCOR1 (Homo sapiens)
MCF-7
Method: immunoprecipitation
Attachment from submitter
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: MCF-7 using the antibody sc-30189. The image shows western blot analysis of input, flowthrough, immunoprecipitate, and mock immunoprecipitate using IgG. Target molecular weight: 53.028.
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
RCOR1 (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line K562 using the antibody sc-30189. Lane 1: input nuclear lysate. Lane 2: material immunoprecipitated with antibody. Lane 3: material immunoprecipitated using control IgG. Marked bands were excised from gel and subjected to analysis by mass spectrometry. Target molecular weight: 53.028.
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
Download
RCOR1 (Homo sapiens)
Method: immunoprecipitation followed by mass spectrometry
compliant
Caption
IP followed by mass spectrometry. Briefly, protein was immunoprecipitated from K562 nuclear cell lysates using the antibody sc-30189, and the IP fraction was loaded on a 10% polyacrylamide gel (NuPAGEBis-Tris Gel) and separated with an Invitrogen NuPAGE electrophoresis system. The gel was stained by ColloidialCoomassie G-250 stain, gel fragments corresponding to the bands indicated were excised. Then proteins were trypsinized using the in-gel digestion method. Digested proteins were analyzed on an Orbitrap Elite mass spectrometer (Thermo Scientific) by the nanoLC-ESI-MS/MS technique. Peptides were identified by the SEQUEST algorithm and filtered with a high confidence threshold (Peptide false discovery rate < 1%, 2 unique peptides per protein minimum, mass error < 10 ppm).
Submitter comment
KDM1A is component of a BHC histone deacetylase complex that contains HDAC1, HDAC2, HMG20B, KDM1A, RCOR1 and PHF21A, http://www.genecards.org/cgi-bin/carddisp.pl?gene=RCOR1&keywords=rcor1. biogrid 21 publication, http://thebiogrid.org/116796/summary/homo-sapiens/rcor1.html
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford