ENCAB823XVS
Antibody against Homo sapiens SUZ12
Homo sapiens
K562, HEK293
characterized to standards
- Status
- released
- Source (vendor)
- Active Motif
- Product ID
- 39357
- Lot ID
- 23808001
- Characterized targets
- SUZ12 (Homo sapiens)
- Host
- rabbit
- Clonality
- polyclonal
- Purification
- affinity
- Isotype
- IgG
- Antigen description
- Sites of PcG activity. This Suz12 antibody was raised against a peptide corresponding to the C-terminus of human Suz12.
- Aliases
- bradley-bernstein:PchAb 166-V
- External resources
Characterizations
SUZ12 (Homo sapiens)
K562HEK293
Method: immunoblot
compliant
- Caption
- 1X10e5 cells equivalent of nuclear extract from HEK293 and K562 cells, were resolved by electrophoresis on a 4-12% acrylamide gel. After separation, the samples were transferred to a nitrocellulose membrane with an Invitrogen iBlot system. Membrane was blocked for an hour in room temperature, with 5% nonfat dry milk and blotted with primary antibody in the appropriate concentration over night at 4c. Membrane was washed and blotted with secondary HRP-conjugated antibody. Detection was made with Optiblot ECL Detect Kit (ab133406) for 2 min. a single band of the expected size was detected (~95kDa).
- Submitted by
- Noam Shoresh
- Lab
- Bradley Bernstein, Broad
- Grant
- U54HG006991
SUZ12 (Homo sapiens)
Method: ChIP-seq comparison
compliant
- Caption
- This validation relies on the use of antibodies to different members of a known complex, and the demonstration that highly similar patterns of enrichment are obtained with each antibody. The second track shown used an antibody to EZH2 (PchAb 58-V), another member of the PRC2 complex. Overall correlation score: 0.9959.
- Submitted by
- Kathrina Onate
- Lab
- Bradley Bernstein, Broad
- Grant
- U54HG006991
- Download
- SUZ12_AM_39357_lot_23808001_SAV.pdf