1. Antibodies
  2. Homo sapiens
  3. XRCC3

ENCAB805XGD

Antibody against Homo sapiens XRCC3

Homo sapiens
K562
characterized to standards
Status
released
Source (vendor)
Active Motif
Product ID
61731
Lot ID
17015001
Characterized targets
XRCC3 (Homo sapiens)
Host
rabbit
Clonality
polyclonal
Isotype
IgG
Aliases
michael-snyder:AS-880
External resources

Characterizations

XRCC3 (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: K562 using the antibody 61731. The image shows western blot analysis of input, flowthrough, immunoprecipitate, and mock immunoprecipitate using IgG. Target molecular weight: 37.850.
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
Download
 11.jpg
XRCC3 (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line K562 using the antibody 61731. Lane 1: input nuclear lysate. Lane 2: material immunoprecipitated with antibody. Lane 3: material immunoprecipitated using control IgG. Marked bands were excised from gel and subjected to analysis by mass spectrometry. Target molecular weight: 37.850.
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
Download
 XRCC3_61731.jpg
XRCC3 (Homo sapiens)
Method: immunoprecipitation followed by mass spectrometry
Attachment PDF Icon
compliant
Caption
IP followed by mass spectrometry. Briefly, protein was immunoprecipitated from K562 nuclear cell lysates using the antibody 61731, and the IP fraction was loaded on a 10% polyacrylamide gel (NuPAGEBis-Tris Gel) and separated with an Invitrogen NuPAGE electrophoresis system. The gel was stained by ColloidialCoomassie G-250 stain, gel fragments corresponding to the bands indicated were excised. Then proteins were trypsinized using the in-gel digestion method. Digested proteins were analyzed on an Orbitrap Elite mass spectrometer (Thermo Scientific) by the nanoLC-ESI-MS/MS technique. Peptides were identified by the SEQUEST algorithm and filtered with a high confidence threshold (Peptide false discovery rate < 1%, 2 unique peptides per protein minimum, mass error < 10 ppm).
Submitter comment
None of the proteins ranked above or equally ranked have been shown to be sequence-specific TFs (including TYY2).
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford