ENCAB801ODE

Antibody against Homo sapiens STAT1

Homo sapiens
HEK293T, HeLa-S3, GM12878
characterized to standards with exemption
Homo sapiens
MCF-7
not characterized to standards
Status
released
Source (vendor)
Santa Cruz Biotech
Product ID
sc-345
Lot ID
H1009
Characterized targets
STAT1 (Homo sapiens)
Host
rabbit
Clonality
polyclonal
Isotype
IgG
Antigen description
epitope mapping at the C-terminus of Stat1α p91 of human origin
Aliases
michael-snyder:AS-195
External resources

Characterizations

STAT1 (Homo sapiens)
GM12878
Method: immunoprecipitation
Attachment from submitter
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: GM12878 using the antibody sc-345. The image shows western blot analysis of input, flowthrough, immunoprecipitate, and mock immunoprecipitate using IgG. Target molecular weight: 91.
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
STAT1 (Homo sapiens)
HeLa-S3
Method: immunoprecipitation
Attachment from submitter
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: HeLa-S3 using the antibody sc-345. The image shows western blot analysis of input, flowthrough, immunoprecipitate, and mock immunoprecipitate using IgG. Target molecular weight: 91.
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
STAT1 (Homo sapiens)
MCF-7
Method: immunoprecipitation
Attachment from submitter
not compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: MCF-7, using the antibody sc-345. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.
Reviewer comment
Multiple bands with none comprising of >50% of signal at expected size. Lanes and marker also not well labelled.
Submitted by
Denis Salins
Lab
Michael Snyder, Stanford
STAT1 (Homo sapiens)
Method: knockdown or knockout
Attachment from submitter
exempt from standards
Caption
A new secondary characterization is not required as one exists for ENCAB000ALF which is a lot of the same antibody (sc-345). The primary characterization of HeLa-S3 (https://www.encodeproject.org/antibody-characterizations/8669e1b5-2b45-4161-bb3a-8018e9b50deb/) and GM12878 (https://www.encodeproject.org/antibody-characterizations/9bfd88f0-ba04-4a73-b496-45a211139494/) for this lot shows the same banding pattern as the primary characterization (https://www.encodeproject.org/antibody-characterizations/dfb157c6-c237-4ef7-ade2-a793e9a28a3e/).
Submitter comment
This is a new lot of a previously characterized antibody ENCAB000ALF and we think no further characterization is necessary beyond the primary if the banding pattern is consistent with the old.
Reviewer comment
This antibody was exempted from secondary characterization by the ENCODE antibody review panel on October 17, 2016.
Submitted by
Jessika Adrian
Lab
Michael Snyder, Stanford
STAT1 (Homo sapiens)
HEK293T
Method: immunoprecipitation
Attachment from submitter
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: HEK293T, using the antibody sc-345. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 91
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford