ENCAB800OND

Alternate accession: ENCAB408SGF

Antibody against Homo sapiens NFE2L2

Homo sapiens
A549, GM12878, HepG2, HeLa-S3, IMR-90
characterized to standards
Homo sapiens
K562
partially characterized
Status
released
Source (vendor)
Santa Cruz Biotech
Product ID
sc-13032
Lot ID
A1711
Characterized targets
NFE2L2 (Homo sapiens)
Lot ID aliases
I1311
Host
rabbit
Clonality
polyclonal
Isotype
IgG
Antigen description
epitope corresponding to amino acids 37-336 mapping near the N-terminus of Nrf2 of human origin
Aliases
michael-snyder:NFE2L2-human_sc-13032_A1711, michael-snyder:AS-973
External resources

Characterizations

NFE2L2 (Homo sapiens)
A549
Method: immunoprecipitation
Attachment from submitter
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: A549 using the antibody sc-13032. The image shows western blot analysis of input, flowthrough, immunoprecipitate, and mock immunoprecipitate using IgG. Target molecular weight: 67.827.
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
NFE2L2 (Homo sapiens)
HeLa-S3
Method: immunoprecipitation
Attachment from submitter
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: HeLa-S3 using the antibody sc-13032. The image shows western blot analysis of input, flowthrough, immunoprecipitate, and mock immunoprecipitate using IgG. Target molecular weight: 67.827.
Submitter comment
NFE2L2 has three isoforms, 68, 66 and 65KD.
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
NFE2L2 (Homo sapiens)
IMR-90
Method: immunoprecipitation
Attachment from submitter
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: IMR90 using the antibody sc-13032. The image shows western blot analysis of input, flowthrough, immunoprecipitate, and mock immunoprecipitate using IgG. Target molecular weight: 67.827.
Reviewer comment
Multiple bands but marked band is >50% of total signal in lane.
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
NFE2L2 (Homo sapiens)
HepG2
Method: immunoprecipitation
Attachment from submitter
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: HepG2 using the antibody sc-13032. The image shows western blot analysis of input, flowthrough, immunoprecipitate, and mock immunoprecipitate using IgG. Target molecular weight: 67.827.
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
NFE2L2 (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: K562 using the antibody sc-13032. The image shows western blot analysis of input, flowthrough, immunoprecipitate, and mock immunoprecipitate using IgG. Target molecular weight: 67.827.
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
NFE2L2 (Homo sapiens)
Method: motif enrichment
compliant
Caption
The motif for target NFE2L2 is represented by the attached position weight matrix (PWM) derived from ENCFF866SGX. Motif enrichment analysis was done by Dr. Zhizhuo Zhang (Broad Institute, Kellis Lab). Accept probability score: 0.716227444 Global enrichment Z-score: 7.788472899 Positional bias Z-score: 11.47707363 Peak rank bias Z-score: 9.50268261 Enrichment rank: 1.0
Submitted by
Kathrina Onate
Lab
Michael Snyder, Stanford
NFE2L2 (Homo sapiens)
GM12878K562HeLa-S3HepG2
Method: immunoblot
Attachment from submitter
compliant
Caption
Western blot analysis of nuclear lysates prepared from multiple cells lines loaded in the order: GM12878, K562, HeLaS3, HepG2 using the antibody sc-13032x. Molecular Weight: 67.827
Reviewer comment
Multiple bands in lanes 2-4 that need to be explained with a suitable reference or confirmed as intended target with a knockdown characterization.
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford