ENCAB797CMZ
Antibody against Homo sapiens ZZZ3
Homo sapiens
K562, MCF-7
characterized to standards with exemption
Homo sapiens
GM12878, HepG2
partially characterized
- Status
- released
- Source (vendor)
- Bethyl Labs
- Product ID
- A303-331A
- Lot ID
- 1
- Characterized targets
- ZZZ3 (Homo sapiens)
- Host
- rabbit
- Clonality
- polyclonal
- Purification
- affinity
- Aliases
- michael-snyder:AS-1434
- External resources
Characterizations
ZZZ3 (Homo sapiens)
K562
not compliant
- Caption
- Immunoprecipitation was performed on nuclear extracts from the cell line: K562, using the antibody A303-331A. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 102.023
- Reviewer comment
- Not readily interpretable, but looks to be multiple bands where the marked band is not >50% of the total signal in the lane.
- Submitted by
- Denis Salins
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG006996
ZZZ3 (Homo sapiens)
K562
exempt from standards
- Caption
- Immunoprecipitation was performed on nuclear extracts from the cell line K562 using the antibody A303-331A. Lane 1: input nuclear lysate. Lane 2: material immunoprecipitated with antibody. Lane 3: material immunoprecipitated using control IgG. Marked bands were excised from gel and subjected to analysis by mass spectrometry. Target molecular weight: 102.023.
- Submitter comment
- We detected ZZZ3 in the marked immunoreactive bands by mass spec.
- Reviewer comment
- ZZZ3 was detected in both bands A and B in associated mass-spec.
- Submitted by
- Nathaniel Watson
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG006996
- Download
- MS1038_3_ZZZ3-A303-331A-1.jpg
ZZZ3 (Homo sapiens)
exempt from standards
- Caption
- IP followed by mass spectrometry. Briefly, protein was immunoprecipitated from K562 nuclear cell lysates using the antibody A303-331A, and the IP fraction was loaded on a 10% polyacrylamide gel (NuPAGEBis-Tris Gel) and separated with an Invitrogen NuPAGE electrophoresis system. The gel was stained by ColloidialCoomassie G-250 stain, gel fragments corresponding to the bands indicated were excised. Then proteins were trypsinized using the in-gel digestion method. Digested proteins were analyzed on an Orbitrap Elite mass spectrometer (Thermo Scientific) by the nanoLC-ESI-MS/MS technique. Peptides were identified by the SEQUEST algorithm and filtered with a high confidence threshold (Peptide false discovery rate < 1%, 2 unique peptides per protein minimum, mass error < 10 ppm).
- Submitter comment
- LUZP1, SUN2, C3, CSRP2BP, ALB, TTR and HSPA1A are not TFs. YEATS2 has interaction with ZZZ3, https://thebiogrid.org/117482/summary/homo-sapiens/zzz3.html.
- Reviewer comment
- The submitters have provided references showing interactions between ZZZ3 and YEATS2, which was ranked higher than ZZZ3 in the ranked peptide list.
- Submitted by
- Nathaniel Watson
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG006996
- Download
- ZZZ3_A303-331A final.pdf
ZZZ3 (Homo sapiens)
GM12878
not submitted for review by lab
- Caption
- Immunoprecipitation was performed on nuclear extracts from the cell line: GM12878 using the antibody A303-331A. The image shows western blot analysis of input, flowthrough, immunoprecipitate, and mock immunoprecipitate using IgG. Target molecular weight: 102.023.
- Submitted by
- Nathaniel Watson
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG006996
- Download
- ZZZ3_A303-331A_GM12878.jpg
ZZZ3 (Homo sapiens)
HepG2
not submitted for review by lab
- Caption
- Immunoprecipitation was performed on nuclear extracts from the cell line: HepG2 using the antibody A303-331A. The image shows western blot analysis of input, flowthrough, immunoprecipitate, and mock immunoprecipitate using IgG. Target molecular weight: 102.023.
- Submitted by
- Nathaniel Watson
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG006996
- Download
- ZZZ3_A303-331A_HepG2.jpg
ZZZ3 (Homo sapiens)
MCF-7
exempt from standards
- Caption
- Immunoprecipitation was performed on nuclear extracts from the cell line: MCF-7 using the antibody A303-331A. The image shows western blot analysis of input, flowthrough, immunoprecipitate, and mock immunoprecipitate using IgG. Target molecular weight: 102.023.
- Submitter comment
- ZZZ3 was detected in both bands A and B by mass spec in K562.
- Reviewer comment
- Marked band is not within 20% of the expected size but is consistent with the band used in mass-spec analysis in K562, which detected ZZZ3.
- Submitted by
- Nathaniel Watson
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG006996
- Download
- ZZZ3_A303-331A_MCF7.jpg