ENCAB797CMZ

Antibody against Homo sapiens ZZZ3

Homo sapiens
MCF-7, K562
characterized to standards with exemption
Homo sapiens
HepG2, GM12878
partially characterized
Status
released
Source (vendor)
Bethyl Labs
Product ID
A303-331A
Lot ID
1
Characterized targets
ZZZ3 (Homo sapiens)
Host
rabbit
Clonality
polyclonal
Purification
affinity
Aliases
michael-snyder:AS-1434
External resources

Characterizations

ZZZ3 (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
not compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: K562, using the antibody A303-331A. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 102.023
Reviewer comment
Not readily interpretable, but looks to be multiple bands where the marked band is not >50% of the total signal in the lane.
Submitted by
Denis Salins
Lab
Michael Snyder, Stanford
ZZZ3 (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
exempt from standards
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line K562 using the antibody A303-331A. Lane 1: input nuclear lysate. Lane 2: material immunoprecipitated with antibody. Lane 3: material immunoprecipitated using control IgG. Marked bands were excised from gel and subjected to analysis by mass spectrometry. Target molecular weight: 102.023.
Submitter comment
We detected ZZZ3 in the marked immunoreactive bands by mass spec.
Reviewer comment
ZZZ3 was detected in both bands A and B in associated mass-spec.
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
ZZZ3 (Homo sapiens)
Method: immunoprecipitation followed by mass spectrometry
exempt from standards
Caption
IP followed by mass spectrometry. Briefly, protein was immunoprecipitated from K562 nuclear cell lysates using the antibody A303-331A, and the IP fraction was loaded on a 10% polyacrylamide gel (NuPAGEBis-Tris Gel) and separated with an Invitrogen NuPAGE electrophoresis system. The gel was stained by ColloidialCoomassie G-250 stain, gel fragments corresponding to the bands indicated were excised. Then proteins were trypsinized using the in-gel digestion method. Digested proteins were analyzed on an Orbitrap Elite mass spectrometer (Thermo Scientific) by the nanoLC-ESI-MS/MS technique. Peptides were identified by the SEQUEST algorithm and filtered with a high confidence threshold (Peptide false discovery rate < 1%, 2 unique peptides per protein minimum, mass error < 10 ppm).
Submitter comment
LUZP1, SUN2, C3, CSRP2BP, ALB, TTR and HSPA1A are not TFs. YEATS2 has interaction with ZZZ3, https://thebiogrid.org/117482/summary/homo-sapiens/zzz3.html.
Reviewer comment
The submitters have provided references showing interactions between ZZZ3 and YEATS2, which was ranked higher than ZZZ3 in the ranked peptide list.
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
ZZZ3 (Homo sapiens)
GM12878
Method: immunoprecipitation
Attachment from submitter
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: GM12878 using the antibody A303-331A. The image shows western blot analysis of input, flowthrough, immunoprecipitate, and mock immunoprecipitate using IgG. Target molecular weight: 102.023.
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
ZZZ3 (Homo sapiens)
HepG2
Method: immunoprecipitation
Attachment from submitter
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: HepG2 using the antibody A303-331A. The image shows western blot analysis of input, flowthrough, immunoprecipitate, and mock immunoprecipitate using IgG. Target molecular weight: 102.023.
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
ZZZ3 (Homo sapiens)
MCF-7
Method: immunoprecipitation
Attachment from submitter
exempt from standards
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: MCF-7 using the antibody A303-331A. The image shows western blot analysis of input, flowthrough, immunoprecipitate, and mock immunoprecipitate using IgG. Target molecular weight: 102.023.
Submitter comment
ZZZ3 was detected in both bands A and B by mass spec in K562.
Reviewer comment
Marked band is not within 20% of the expected size but is consistent with the band used in mass-spec analysis in K562, which detected ZZZ3.
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford