ENCAB796NDK

Antibody against Homo sapiens ARID1B

Homo sapiens
K562
characterized to standards with exemption
Status
released
Source (vendor)
Bethyl Labs
Product ID
A301-046A
Lot ID
2
Characterized targets
ARID1B (Homo sapiens)
Host
rabbit
Clonality
polyclonal
Purification
affinity
Aliases
michael-snyder:AS-1484
External resources

Characterizations

ARID1B (Homo sapiens)
Method: immunoprecipitation followed by mass spectrometry
compliant
Caption
IP followed by mass spectrometry. Briefly, protein was immunoprecipitated from K562 nuclear cell lysates using the antibody A301-046A, and the IP fraction was loaded on a 10% polyacrylamide gel (NuPAGEBis-Tris Gel) and separated with an Invitrogen NuPAGE electrophoresis system. The gel was stained by ColloidialCoomassie G-250 stain, gel fragments corresponding to the bands indicated were excised. Then proteins were trypsinized using the in-gel digestion method. Digested proteins were analyzed on an Orbitrap Elite mass spectrometer (Thermo Scientific) by the nanoLC-ESI-MS/MS technique. Peptides were identified by the SEQUEST algorithm and filtered with a high confidence threshold (Peptide false discovery rate < 1%, 2 unique peptides per protein minimum, mass error < 10 ppm).
Submitter comment
ARID1B, SMARCE1, SMARCD2, SMARCC1, ACTL6A, SMARCD1, SMARCB1 and SMARCC2 are components of SWI/SNF complex, http://www.genecards.org/cgi-bin/carddisp.pl?gene=ARID1B&keywords=arid1b. ARID1B and DPF2 have interaction, https://thebiogrid.org/121559/summary/homo-sapiens/arid1b.html.
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
ARID1B (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
not compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: K562, using the antibody A301-046A. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 236.123
Reviewer comment
bad IgG smear, multiple bands
Submitted by
Denis Salins
Lab
Michael Snyder, Stanford
ARID1B (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
exempt from standards
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line K562 using the antibody A301-046A. Lane 1: input nuclear lysate. Lane 2: material immunoprecipitated with antibody. Lane 3: material immunoprecipitated using control IgG. Marked bands were excised from gel and subjected to analysis by mass spectrometry. Target molecular weight: 236.123.
Submitter comment
Multiple immunoreactive bands (some outside of the 20% allowed deviation) were detected and analyzed by mass spec.
Reviewer comment
ARID1B was detected in band B (at unexpected size) by mass spec
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford