1. Antibodies
  2. Homo sapiens
  3. HNRNPM

ENCAB792TQV

Antibody against Homo sapiens HNRNPM

Homo sapiens
K562, HepG2
characterized to standards
Homo sapiens
any cell type or tissue
partially characterized
Status
released
Source (vendor)
Santa Cruz Biotech
Product ID
sc-20001
Lot ID
D1304
Characterized targets
HNRNPM (Homo sapiens)
Host
mouse
Clonality
monoclonal
Purification
affinity
Isotype
IgG1
Antigen description
hnRNP M3,4 (2A6) is a mouse monoclonal antibody raised against fusion protein consisting of entire M4 protein sequence.
Aliases
xiang-dong-fu:hnRNPM
External resources

Characterizations

HNRNPM (Homo sapiens)
HepG2
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
compliant
Caption
IP-Western Blot analysis of HepG2 whole cell lysate using HNRNPM specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using mouse polyclonal anti-HNRNPM antibody (lanes under 'HNRNPM').
Submitted by
Steven Blue
Lab
Gene Yeo, UCSD
HNRNPM (Homo sapiens)
Method: knockdown or knockout
Attachment from submitter
Attachment from submitters
compliant
Caption
Western blot following shRNA against HNRNPM in K562 and HepG2 whole cell lysate using HNRNPM specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against HNRNPM. Lanes 5-8 follow the same pattern, but in HepG2. HNRNPM protein appears as the green band, Tubulin serves as a control and appears in red.
Submitted by
Xintao Wei
Lab
Brenton Graveley, UConn
HNRNPM (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
compliant
Caption
IP-Western Blot analysis of K562 whole cell lysate using HNRNPM specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using mouse normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using mouse monoclonal anti-HNRNPM antibody (lanes under 'HNRNPM').
Submitted by
Balaji Sundararaman
Lab
Gene Yeo, UCSD
HNRNPM (Homo sapiens)
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
not submitted for review by lab
Caption
IP-WB characterization of HNRNPM specific antibody in K562 cell line . Lane 1 is 2.5% of five million K562 whole cell lysate Input, lane 2 is 2.5% of supernatant after immunoprecipitation and Lane 3 is 50% of IP enrichment using mouse monoclonal hnRNP M3/4 (2A6). This antibody passes preliminary validation and will be further pursued for primary and secondary validation.
Submitted by
Balaji Sundararaman
Lab
Gene Yeo, UCSD