1. Antibodies
  2. Homo sapiens
  3. RNF2

ENCAB790JUW

Alternate accessions: ENCAB000AKK, ENCAB729RXO

Antibody against Homo sapiens RNF2

Homo sapiens
K562
characterized to standards
Homo sapiens
H1, HeLa-S3
characterized to standards with exemption
Status
released
Source (vendor)
Bethyl Labs
Product ID
A302-869A
Lot ID
1
Characterized targets
RNF2 (Homo sapiens)
Host
rabbit
Clonality
polyclonal
Purification
affinity
Aliases
michael-snyder:AS-1278, bradley-bernstein:PchAb 155
External resources

Characterizations

RNF2 (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: K562, using the antibody A302-869A. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.
Submitted by
Denis Salins
Lab
Michael Snyder, Stanford
RNF2 (Homo sapiens)
Method: ChIP-string comparison
Attachment from submitter
Attachment from submitters
not reviewed
Submitted by
Charles Epstein
Lab
Bradley Bernstein, Broad
RNF2 (Homo sapiens)
H1HeLa-S3
Method: immunoblot
Attachment from submitter
Attachment from submitters
exempt from standards
Submitter comment
The antibody review board chose to exempt this for H1-hESC and HeLaS3 given the K562 results and the age of this data.
Reviewer comment
Confirmed that this was given an exemption for H1-HESC given the results of K562.
Submitted by
Charles Epstein
Lab
Bradley Bernstein, Broad
RNF2 (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line K562 using the antibody A302-869A. Lane 1: input nuclear lysate. Lane 2: material immunoprecipitated with antibody. Lane 3: material immunoprecipitated using control IgG. Marked bands were excised from gel and subjected to analysis by mass spectrometry. Target molecular weight: 37.655.
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
RNF2 (Homo sapiens)
Method: immunoprecipitation followed by mass spectrometry
Attachment PDF Icon
compliant
Caption
IP followed by mass spectrometry. Briefly, protein was immunoprecipitated from K562 nuclear cell lysates using the antibody A302-869A, and the IP fraction was loaded on a 10% polyacrylamide gel (NuPAGEBis-Tris Gel) and separated with an Invitrogen NuPAGE electrophoresis system. The gel was stained by ColloidialCoomassie G-250 stain, gel fragments corresponding to the bands indicated were excised. Then proteins were trypsinized using the in-gel digestion method. Digested proteins were analyzed on an Orbitrap Elite mass spectrometer (Thermo Scientific) by the nanoLC-ESI-MS/MS technique. Peptides were identified by the SEQUEST algorithm and filtered with a high confidence threshold (Peptide false discovery rate < 1%, 2 unique peptides per protein minimum, mass error < 10 ppm).
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford