1. Antibodies
  2. Homo sapiens
  3. BRD4

ENCAB782ZNQ

Antibody against Homo sapiens BRD4

Homo sapiens
K562, HepG2, GM12878, endothelial cell of umbilical vein
characterized to standards
Status
released
Source (vendor)
Bethyl Labs
Product ID
A301-985A50
Lot ID
3
Characterized targets
BRD4 (Homo sapiens)
Host
rabbit
Clonality
polyclonal
Purification
affinity
Isotype
IgG
Antigen description
The epitope recognized by A301-985A50 maps to a region between residue 1312 and 1362 of human bromodomain-containing protein 4 using the numbering given in entry NP_490597.1 (GeneID 23476).
Aliases
bradley-bernstein:PchAb 1271
External resources

Characterizations

BRD4 (Homo sapiens)
Method: ChIP-seq comparison
Attachment PDF Icon
compliant
Caption
This validation relies on the use of antibodies to a chromatin regulator (BRD4) and a histone modification (H3K27ac) in K562 cells, and the demonstration that highly similar patterns of enrichment are obtained with each antibody. The first track shown used an antibody to BRD4 (PchAb 1271, ENCAB782ZNQ), and the second track shown used an antibody to H3K27ac previously characterized to Encode standards (PchAb 205, ENCAB000AQN). Overall correlation score = 0.8046
Submitter comment
SAV2
Submitted by
Nina Farrell
Lab
Bradley Bernstein, Broad
BRD4 (Homo sapiens)
K562HepG2GM12878endothelial cell of umbilical vein
Method: immunoblot
Attachment from submitter
Attachment from submitters
compliant
Caption
Nuclear lysates from K562 (10ug), HepG2 (7ug), GM12878 (10ug), HUVEC (10ug), were loaded into a 3-8% TA gel in 1X TA running buffer. After separation, the samples were transferred to a nitrocellulose membrane with an Invitrogen iBlot system. Membrane was blocked for an hour in room temperature, with 5% BSA in TBS-T and blotted with primary antibody in the appropriate concentration over night at 4c. Membrane was washed and blotted with secondary HRP-conjugated antibody. Detection was made with Optiblot ECL Detect Kit (ab133406) for 2 min. a band of the expected size was detected in all cell types.
Submitted by
Noam Shoresh
Lab
Bradley Bernstein, Broad