ENCAB778TBN
Antibody against Homo sapiens H3K9me3
Homo sapiens
any cell type or tissue
characterized to standards with exemption
- Status
- released
- Source (vendor)
- Abcam
- Product ID
- ab8898
- Lot ID
- GR93671-1
- Characterized targets
- H3K9me3 (Homo sapiens)
- Host
- rabbit
- Clonality
- polyclonal
- Aliases
- bradley-bernstein:PchAb 911
- External resources
Characterizations
H3K9me3 (Homo sapiens)
K562
Method: immunoblot
exempt from standards
- Caption
- This particular lot of this antibody was exhausted before characterizations were made. The same antibody was characterized in ENCAB000AUR.
- Submitter comment
- Situation: This antibody is the same manufacturer part number as the H3K9me3 antibody ENCAB000AUR, just a different lot number. This antibody lacks a primary validation. However, we have submitted a ChIP-ChIP comparison analysis of this antibody with another H3K9me3 antibody already characterized to standards in epiblast cells. The analysis shows highly similar patterns of enrichment obtained with each antibody. We note that due to the relatively limited coverage of H3K9me3 in non-repetitive regions of the genome, we expected a lower track-track correlation for H3K9me3 than for other more ubiquitously occurring peaks. We are asking for the secondary validation to be exempted from standard for this reason. We have no remaining inventory of the antibody in our lab.
- Reviewer comment
- PJF: I agree that the ChIP-seq comparison is sufficient and that the antibody should be released as “characterized to standard with exemption”.
- Submitted by
- Nina Farrell
- Lab
- Bradley Bernstein, Broad
- Grant
- U54HG006991
- Download
- exempted.png
H3K9me3 (Homo sapiens)
Method: ChIP-seq comparison
exempt from standards
- Caption
- This validation relies on the use of antibodies to a histone modification (H3K9me3) in epiblast cells, and the demonstration that highly similar patterns of enrichment are obtained with each antibody. Please note that the two antibodies originate from two lots of the same vendor antibody part number. The first track shown used an antibody to H3K9me3 (PchAb 911, ENCAB778TBN), and the second track shown used an antibody to H3K9me3 previously characterized to Encode standards (PchAb 125- V, ENCAB000APY). Genomewide correlation = 0.72; p-value < 2.2E-16. Please note, due to the relatively limited coverage of H3K9me3 in non-repetitive regions of the genome, we expect a lower track – track correlation for H3K9me3 than for other more ubiquitously occurring peaks.
- Submitter comment
- Situation: This antibody is the same manufacturer part number as the H3K9me3 antibody above, just a different lot number. This antibody lacks a primary validation. However, we have submitted a ChIP-ChIP comparison analysis of this antibody with another H3K9me3 antibody already characterized to standards in epiblast cells. The analysis shows highly similar patterns of enrichment obtained with each antibody. We note that due to the relatively limited coverage of H3K9me3 in non-repetitive regions of the genome, we expected a lower track-track correlation for H3K9me3 than for other more ubiquitously occurring peaks. We are asking for the secondary validation to be exempted from standard for this reason. We have no remaining inventory of the antibody in our lab.
- Reviewer comment
- PJF: I agree that the ChIP-seq comparison is sufficient and that the antibody should be released as ““characterized to standard with exemption”
- Submitted by
- Nina Farrell
- Lab
- Bradley Bernstein, Broad
- Grant
- U54HG006991
- Download
- H3K9me3 PchAb 911 SAV.pdf