ENCAB778OCV

Antibody against Homo sapiens ELF1

Homo sapiens
MCF-7, GM12878, K562, HepG2
characterized to standards with exemption
Status
released
Source (vendor)
Bethyl Labs
Product ID
A301-443A
Lot ID
1
Characterized targets
ELF1 (Homo sapiens)
Host
rabbit
Clonality
polyclonal
Purification
affinity
Aliases
michael-snyder:AS-1249
External resources

Characterizations

ELF1 (Homo sapiens)
Method: immunoprecipitation followed by mass spectrometry
compliant
Caption
IP followed by mass spectrometry. Briefly, protein was immunoprecipitated from K562 nuclear cell lysates using the antibody A301-443A, and the IP fraction was loaded on a 10% polyacrylamide gel (NuPAGEBis-Tris Gel) and separated with an Invitrogen NuPAGE electrophoresis system. The gel was stained by ColloidialCoomassie G-250 stain, gel fragments corresponding to the bands indicated were excised. Then proteins were trypsinized using the in-gel digestion method. Digested proteins were analyzed on an Orbitrap Elite mass spectrometer (Thermo Scientific) by the nanoLC-ESI-MS/MS technique. Peptides were identified by the SEQUEST algorithm and filtered with a high confidence threshold (Peptide false discovery rate < 1%, 2 unique peptides per protein minimum, mass error < 10 ppm).
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
ELF1 (Homo sapiens)
Method: motif enrichment
compliant
Caption
The motif for target ELF1 is represented by the attached position weight matrix (PWM) derived from ENCFF163MCL Motif enrichment analysis was done by Dr. Zhizhuo Zhang (Broad Institute, Kellis Lab). Accept probability score: 0.730876793 Global enrichment Z-score: 6.801200187 Positional bias Z-score: 10.51694949 Peak rank bias Z-score: 10.31905041 Enrichment rank: 1.0
Submitted by
Kathrina Onate
Lab
Michael Snyder, Stanford
ELF1 (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
not compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: K562, using the antibody A301-443A. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 67.498
Reviewer comment
The band they point to is not strong, nor is it near the 75 KD mark
Submitted by
Denis Salins
Lab
Michael Snyder, Stanford
ELF1 (Homo sapiens)
HepG2
Method: immunoprecipitation
Attachment from submitter
exempt from standards
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: HepG2 using the antibody A301-443A. The image shows western blot analysis of input, flowthrough, immunoprecipitate, and mock immunoprecipitate using IgG. Target molecular weight: 67.498.
Submitter comment
1) same pattern as K562 IP. 2) Mass Spec result indicates that the both bands(~75-100kd) are ELF1
Reviewer comment
Not within 20% of expected size but is consistent with banding pattern in K562 which was analyzed by mass spec and confirmed to detect ELF1.
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
ELF1 (Homo sapiens)
MCF-7
Method: immunoprecipitation
Attachment from submitter
exempt from standards
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: MCF-7 using the antibody A301-443A. The image shows western blot analysis of input, flowthrough, immunoprecipitate, and mock immunoprecipitate using IgG. Target molecular weight: 67.498.
Submitter comment
1) same pattern as K562 IP. 2) Mass Spec result indicates that the both bands(~75-100kd) are ELF1
Reviewer comment
Not within 20% of expected size but is consistent with banding pattern in K562 which was analyzed by mass spec and confirmed to detect ELF1.
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
ELF1 (Homo sapiens)
GM12878
Method: immunoprecipitation
Attachment from submitter
exempt from standards
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: GM12878 using the antibody A301-443A. The image shows western blot analysis of input, flowthrough, immunoprecipitate, and mock immunoprecipitate using IgG. Target molecular weight: 67.498.
Submitter comment
1) same pattern as K562 IP. 2) Mass Spec result indicates that the both bands(~75-100kd) are ELF1
Reviewer comment
Not within 20% of expected size but is consistent with banding pattern in K562 which was analyzed by mass spec and confirmed to detect ELF1.
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
ELF1 (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
exempt from standards
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line K562 using the antibody A301-443A. Lane 1: input nuclear lysate. Lane 2: material immunoprecipitated with antibody. Lane 3: material immunoprecipitated using control IgG. Marked bands were excised from gel and subjected to analysis by mass spectrometry. Target molecular weight: 67.498.
Submitter comment
--
Reviewer comment
Not within 20% of expected size. Rescued by mass spectrometry analysis
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford