ENCAB777CMB
Antibody against Homo sapiens MAK16
Homo sapiens
any cell type or tissue
partially characterized
- Status
- released
- Source (vendor)
- Bethyl Labs
- Product ID
- A301-232A
- Lot ID
- 1
- Characterized targets
- MAK16 (Homo sapiens)
- Host
- rabbit
- Clonality
- polyclonal
- Purification
- affinity
- Isotype
- IgG
- External resources
Characterizations
MAK16 (Homo sapiens)
Method: immunoprecipitation
not submitted for review by lab
- Caption
- IP-WB analysis of K562 whole cell lysate using MAK16 specific antibody. Lane 1 is 2.5% of 0.5mg input lysate, lane 2 is 2.5% of supernatant after immunoprecipitation and Lane 3 is 50% of IP enrichment using rabbit polyclonal RBM13 Antibody. This antibody passes preliminary validation and will be further pursued for primary and secondary validation.
- Submitted by
- Balaji Sundararaman
- Lab
- Gene Yeo, UCSD
- Grant
- U54HG007005
- Download
- Bethyl_A301-232A_1_MAK16.png
MAK16 (Homo sapiens)
Method: knockdown or knockout
compliant
- Caption
- Western blot following CRISPR against MAK16 in HepG2 whole cell lysate using MAK16 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against MAK16. MAK16 protein appears as the green band, Beta-actin serves as a control and appears in red.
- Submitted by
- Xintao Wei
- Lab
- Brenton Graveley, UConn
- Grant
- U41HG009889
- Download
- MAK16-HEPG2-CRISPR.png
MAK16 (Homo sapiens)
Method: knockdown or knockout
compliant
- Caption
- Western blot following shRNA against MAK16 in K562 whole cell lysate using MAK16 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against MAK16.MAK16 protein appears as the green band, Tubulin serves as a control and appears in red.
- Submitted by
- Xintao Wei
- Lab
- Brenton Graveley, UConn
- Grant
- U54HG007005
- Download
- MAK16-k562_Secondary_Western.png