ENCAB749XQY
Antibody against Homo sapiens SMC3
Homo sapiens
A549, IMR-90, HepG2, K562
characterized to standards
- Status
- released
- Source (vendor)
- Abcam
- Product ID
- ab9263
- Lot ID
- GR214360
- Characterized targets
- SMC3 (Homo sapiens)
- Host
- rabbit
- Clonality
- polyclonal
- Purification
- affinity
- Isotype
- IgG
- Aliases
- michael-snyder:AS-1662
- External resources
Characterizations
SMC3 (Homo sapiens)
A549
compliant
- Caption
- Immunoprecipitation was performed on nuclear extracts from the cell line: A549 using the antibody ab9263 (Lot: GR214360). The image shows western blot analysis of input, flowthrough, immunoprecipitate, and mock immunoprecipitate using IgG. Target molecular weight: 141.5.
- Submitted by
- Nathaniel Watson
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG006996
- Download
- #1143 A549 SMC3 ab9263.jpg
SMC3 (Homo sapiens)
HepG2
compliant
- Caption
- Immunoprecipitation was performed on nuclear extracts from the cell line: HepG2 using the antibody ab9263 (Lot: GR214360). The image shows western blot analysis of input, flowthrough, immunoprecipitate, and mock immunoprecipitate using IgG. Target molecular weight: 141.5.
- Submitted by
- Nathaniel Watson
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG006996
- Download
- #1143 HepG2 SMC3(ab9263).jpg
SMC3 (Homo sapiens)
IMR-90
compliant
- Caption
- Immunoprecipitation was performed on nuclear extracts from the cell line: IMR90 using the antibody ab9263 (Lot: GR214360). The image shows western blot analysis of input, flowthrough, immunoprecipitate, and mock immunoprecipitate using IgG. Target molecular weight: 141.5.
- Submitted by
- Nathaniel Watson
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG006996
- Download
- #1143 IMR90 SMC3(ab9263).jpg
SMC3 (Homo sapiens)
K562
compliant
- Caption
- Immunoprecipitation was performed on nuclear extracts from the cell line K562 using the antibody ab9263 (Lot: GR214360). Lane 1: input nuclear lysate. Lane 2: material immunoprecipitated with antibody. Lane 3: material immunoprecipitated using control IgG. Marked bands were excised from gel and subjected to analysis by mass spectrometry. Target molecular weight: 141.5.
- Submitter comment
- Despite multiple immunoreactive bands seen in the IP, the mass-spec characterization confirms the presence of SMC3 in all of them.
- Submitted by
- Nathaniel Watson
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG006996
- Download
- SMC3_ab9263.jpg
SMC3 (Homo sapiens)
compliant
- Caption
- IP followed by mass spectrometry. Briefly, protein was immunoprecipitated from K562 nuclear cell lysates using the antibody ab9263 (Lot: GR214360), and the IP fraction was loaded on a 10% polyacrylamide gel (NuPAGEBis-Tris Gel) and separated with an Invitrogen NuPAGE electrophoresis system. The gel was stained by ColloidialCoomassie G-250 stain, gel fragments corresponding to the bands indicated were excised. Then proteins were trypsinized using the in-gel digestion method. Digested proteins were analyzed on an Orbitrap Elite mass spectrometer (Thermo Scientific) by the nanoLC-ESI-MS/MS technique. Peptides were identified by the SEQUEST algorithm and filtered with a high confidence threshold (Peptide false discovery rate < 1%, 2 unique peptides per protein minimum, mass error < 10 ppm).
- Submitted by
- Nathaniel Watson
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG006996
- Download
- SMC3_ab9263_final.pdf