ENCAB749XQY

Antibody against Homo sapiens SMC3

Homo sapiens
HepG2, A549, IMR-90, K562
characterized to standards
Status
released
Source (vendor)
Abcam
Product ID
ab9263
Lot ID
GR214360
Characterized targets
SMC3 (Homo sapiens)
Host
rabbit
Clonality
polyclonal
Purification
affinity
Isotype
IgG
Aliases
michael-snyder:AS-1662
External resources

Characterizations

SMC3 (Homo sapiens)
A549
Method: immunoprecipitation
Attachment from submitter
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: A549 using the antibody ab9263 (Lot: GR214360). The image shows western blot analysis of input, flowthrough, immunoprecipitate, and mock immunoprecipitate using IgG. Target molecular weight: 141.5.
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
SMC3 (Homo sapiens)
HepG2
Method: immunoprecipitation
Attachment from submitter
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: HepG2 using the antibody ab9263 (Lot: GR214360). The image shows western blot analysis of input, flowthrough, immunoprecipitate, and mock immunoprecipitate using IgG. Target molecular weight: 141.5.
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
SMC3 (Homo sapiens)
IMR-90
Method: immunoprecipitation
Attachment from submitter
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: IMR90 using the antibody ab9263 (Lot: GR214360). The image shows western blot analysis of input, flowthrough, immunoprecipitate, and mock immunoprecipitate using IgG. Target molecular weight: 141.5.
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
SMC3 (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line K562 using the antibody ab9263 (Lot: GR214360). Lane 1: input nuclear lysate. Lane 2: material immunoprecipitated with antibody. Lane 3: material immunoprecipitated using control IgG. Marked bands were excised from gel and subjected to analysis by mass spectrometry. Target molecular weight: 141.5.
Submitter comment
Despite multiple immunoreactive bands seen in the IP, the mass-spec characterization confirms the presence of SMC3 in all of them.
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
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SMC3 (Homo sapiens)
Method: immunoprecipitation followed by mass spectrometry
compliant
Caption
IP followed by mass spectrometry. Briefly, protein was immunoprecipitated from K562 nuclear cell lysates using the antibody ab9263 (Lot: GR214360), and the IP fraction was loaded on a 10% polyacrylamide gel (NuPAGEBis-Tris Gel) and separated with an Invitrogen NuPAGE electrophoresis system. The gel was stained by ColloidialCoomassie G-250 stain, gel fragments corresponding to the bands indicated were excised. Then proteins were trypsinized using the in-gel digestion method. Digested proteins were analyzed on an Orbitrap Elite mass spectrometer (Thermo Scientific) by the nanoLC-ESI-MS/MS technique. Peptides were identified by the SEQUEST algorithm and filtered with a high confidence threshold (Peptide false discovery rate < 1%, 2 unique peptides per protein minimum, mass error < 10 ppm).
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford