ENCAB741YHG

Antibody against Homo sapiens SUZ12

Homo sapiens
endothelial cell of umbilical vein, HepG2
characterized to standards
Homo sapiens
GM12878
characterized to standards with exemption
Status
released
Source (vendor)
Cell Signaling
Product ID
3737BF
Lot ID
4
Characterized targets
SUZ12 (Homo sapiens)
Host
rabbit
Clonality
monoclonal
Antigen description
Sites of PcG activity
Aliases
bradley-bernstein:PchAb 1147
External resources

Characterizations

SUZ12 (Homo sapiens)
GM12878
Method: immunoblot
Attachment from submitter
exempt from standards
Caption
This antibody was used before a characterization for GM12878 was performed. It has been reviewed and exempted by the antibody characterization board.
Submitter comment
The antibody has a conforming western only in HepG2 and HUVEC. A third dataset (https://www.encodeproject.org/experiments/ENCSR091BOQ/) used this antibody in GM12878. Release of that single dataset is blocked due to the lack of primary validation in that cell type. We would like an exception to allow the release of this one dataset. We do not anticipate further use of this antibody and have no remaining inventory.
Reviewer comment
PJF: I agree that this dataset should be released without requiring a western blot in GM12978 (using whatever term is appropriate such as “characterized to standard with exemption”).
Submitted by
Nina Farrell
Lab
Bradley Bernstein, Broad
Download
SUZ12 (Homo sapiens)
Method: ChIP-seq comparison
compliant
Caption
This validation relies on the use of antibodies to different members of a known complex, and the demonstration that highly similar patterns of enrichment are obtained with each antibody. The second track shown used an antibody to EZH2 (PchAb 58-V), another member of the PRC2 complex. Overall correlation score: SUZ12 vs EZH2: 0.9439.
Submitted by
Kathrina Onate
Lab
Bradley Bernstein, Broad
SUZ12 (Homo sapiens)
HepG2endothelial cell of umbilical vein
Method: immunoblot
Attachment from submitter
compliant
Caption
Nuclear lysates from HepG2 (10ug), HUVEC (10ug), were loaded into a 4-12% Bis-Tris gel in 1X MES running buffer. After separation, the samples were transferred to a nitrocellulose membrane using the iblot system. Membrane was blocked for an hour in room temperature, with 5% BSA in TBS-T and blotted with primary antibody in the appropriate concentration over night at 4c. Membrane was washed and blotted with secondary HRP-conjugated antibody. Detection was made with Optiblot ECL Detect Kit (ab133406) for 2 min.A band of the expected size was detected (~83kDa).
Submitted by
Noam Shoresh
Lab
Bradley Bernstein, Broad