ENCAB733KID
Antibody against Homo sapiens BCLAF1
Homo sapiens
K562, HEK293T, HepG2, MCF-7, GM12878
partially characterized
- Status
- released
- Source (vendor)
- Bethyl Labs
- Product ID
- A300-609A
- Lot ID
- 1
- Characterized targets
- BCLAF1 (Homo sapiens)
- Host
- rabbit
- Clonality
- polyclonal
- Purification
- affinity
- Aliases
- michael-snyder:AS-1561
- External resources
Characterizations
BCLAF1 (Homo sapiens)
K562
Method: immunoprecipitation
exempt from standards
- Caption
- Immunoprecipitation was performed on nuclear extracts from the cell line: K562, using the antibody A300-609A. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 106.122
- Submitter comment
- We think the signal runs higher than at the expected size possibly because BCLF1 can have binary interactions with BCL2 (~22-29kD) and EMD (~28kD): http://www.uniprot.org/uniprot/Q9NYF8#interaction See also ENCAB012FUX
- Reviewer comment
- Band not within 20% of expected size, it's ~150kD.
- Submitted by
- Denis Salins
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG006996
- Download
- 1095_08_BTF_A300-609A.jpg
BCLAF1 (Homo sapiens)
HepG2
Method: immunoprecipitation
exempt from standards
- Caption
- Immunoprecipitation was performed on nuclear extracts from the cell line: HepG2, using the antibody A300-609A. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 106.122
- Submitter comment
- We think the signal runs higher than at the expected size possibly because BCLF1 can have binary interactions with BCL2 (~22-29kD) and EMD (~28kD): http://www.uniprot.org/uniprot/Q9NYF8#interaction See also ENCAB012FUX
- Reviewer comment
- Band not within 20% of expected size.
- Submitted by
- Nathaniel Watson
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG006996
- Download
- 1114_11_BTF_A300-609A.jpg
BCLAF1 (Homo sapiens)
HEK293T
Method: immunoprecipitation
exempt from standards
- Caption
- Immunoprecipitation was performed on nuclear extracts from the cell line: HEK293T, using the antibody A300-609A. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 106.122
- Submitter comment
- We think the signal runs higher than at the expected size possibly because BCLF1 can have binary interactions with BCL2 (~22-29kD) and EMD (~28kD): http://www.uniprot.org/uniprot/Q9NYF8#interaction See also ENCAB012FUX
- Reviewer comment
- Band not within 20% of expected size.
- Submitted by
- Nathaniel Watson
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG006996
- Download
- 1114_12_BTF_A300-609A.jpg
BCLAF1 (Homo sapiens)
GM12878
Method: immunoprecipitation
exempt from standards
- Caption
- Immunoprecipitation was performed on nuclear extracts from the cell line: GM12878, using the antibody A300-609A. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 106.122
- Submitter comment
- We think the signal runs higher than at the expected size possibly because BCLF1 can have binary interactions with BCL2 (~22-29kD) and EMD (~28kD): http://www.uniprot.org/uniprot/Q9NYF8#interaction See also ENCAB012FUX
- Reviewer comment
- Band not within 20% of expected size.
- Submitted by
- Nathaniel Watson
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG006996
- Download
- 1116_11_BTF_A300-609A.jpg
BCLAF1 (Homo sapiens)
MCF-7
Method: immunoprecipitation
exempt from standards
- Caption
- Immunoprecipitation was performed on nuclear extracts from the cell line: MCF-7, using the antibody A300-609A. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 106.122
- Submitter comment
- We think the signal runs higher than at the expected size possibly because BCLF1 can have binary interactions with BCL2 (~22-29kD) and EMD (~28kD): http://www.uniprot.org/uniprot/Q9NYF8#interaction. See also ENCAB012FUX
- Reviewer comment
- That sounds reasonable.
- Submitted by
- Nathaniel Watson
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG006996
- Download
- 1116_12_BTF_A300-609A.jpg