ENCAB733KID

Antibody against Homo sapiens BCLAF1

Homo sapiens
K562, HEK293T, HepG2, MCF-7, GM12878
partially characterized
Status
released
Source (vendor)
Bethyl Labs
Product ID
A300-609A
Lot ID
1
Characterized targets
BCLAF1 (Homo sapiens)
Host
rabbit
Clonality
polyclonal
Purification
affinity
Aliases
michael-snyder:AS-1561
External resources

Characterizations

BCLAF1 (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
exempt from standards
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: K562, using the antibody A300-609A. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 106.122
Submitter comment
We think the signal runs higher than at the expected size possibly because BCLF1 can have binary interactions with BCL2 (~22-29kD) and EMD (~28kD): http://www.uniprot.org/uniprot/Q9NYF8#interaction See also ENCAB012FUX
Reviewer comment
Band not within 20% of expected size, it's ~150kD.
Submitted by
Denis Salins
Lab
Michael Snyder, Stanford
BCLAF1 (Homo sapiens)
HepG2
Method: immunoprecipitation
Attachment from submitter
exempt from standards
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: HepG2, using the antibody A300-609A. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 106.122
Submitter comment
We think the signal runs higher than at the expected size possibly because BCLF1 can have binary interactions with BCL2 (~22-29kD) and EMD (~28kD): http://www.uniprot.org/uniprot/Q9NYF8#interaction See also ENCAB012FUX
Reviewer comment
Band not within 20% of expected size.
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
BCLAF1 (Homo sapiens)
HEK293T
Method: immunoprecipitation
Attachment from submitter
exempt from standards
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: HEK293T, using the antibody A300-609A. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 106.122
Submitter comment
We think the signal runs higher than at the expected size possibly because BCLF1 can have binary interactions with BCL2 (~22-29kD) and EMD (~28kD): http://www.uniprot.org/uniprot/Q9NYF8#interaction See also ENCAB012FUX
Reviewer comment
Band not within 20% of expected size.
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
BCLAF1 (Homo sapiens)
GM12878
Method: immunoprecipitation
Attachment from submitter
exempt from standards
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: GM12878, using the antibody A300-609A. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 106.122
Submitter comment
We think the signal runs higher than at the expected size possibly because BCLF1 can have binary interactions with BCL2 (~22-29kD) and EMD (~28kD): http://www.uniprot.org/uniprot/Q9NYF8#interaction See also ENCAB012FUX
Reviewer comment
Band not within 20% of expected size.
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
BCLAF1 (Homo sapiens)
MCF-7
Method: immunoprecipitation
Attachment from submitter
exempt from standards
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: MCF-7, using the antibody A300-609A. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 106.122
Submitter comment
We think the signal runs higher than at the expected size possibly because BCLF1 can have binary interactions with BCL2 (~22-29kD) and EMD (~28kD): http://www.uniprot.org/uniprot/Q9NYF8#interaction. See also ENCAB012FUX
Reviewer comment
That sounds reasonable.
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford