ENCAB730BOO
Antibody against Homo sapiens UPF1
Homo sapiens
K562, HepG2
characterized to standards
Homo sapiens
any cell type or tissue
partially characterized
- Status
- released
- Source (vendor)
- MBLI
- Product ID
- RN108PW
- Lot ID
- 001
- Characterized targets
- UPF1 (Homo sapiens)
- Host
- rabbit
- Clonality
- polyclonal
- External resources
Characterizations
UPF1 (Homo sapiens)
HepG2
compliant
- Caption
- IP-Western Blot analysis of HepG2 whole cell lysate using UPF1 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-UPF1 antibody (lanes under 'UPF1').
- Submitted by
- Steven Blue
- Lab
- Gene Yeo, UCSD
- Grant
- U54HG007005
- Download
- HepG2_MBLI_RN108PW_001_UPF1.png
UPF1 (Homo sapiens)
K562
compliant
- Caption
- IP-Western Blot analysis of K562 whole cell lysate using UPF1 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 10% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-UPF1 antibody (lanes under 'UPF1').
- Submitted by
- Steven Blue
- Lab
- Gene Yeo, UCSD
- Grant
- U54HG007005
- Download
- K562_MBLI_RN108PW_001_UPF1.png
UPF1 (Homo sapiens)
not submitted for review by lab
- Caption
- IP-WB analysis of K562 whole cell lysate using UPF1 specific antibody. Lane 1 is 2.5% of five million whole cell lysate Input, lane 2 is 2.5% of supernatant after immunoprecipitation and Lane 3 is 50% of IP enrichment using rabbit polyclonal Anti-UPF1 pAb. This antibody passes preliminary validation and will be further pursued for primary and secondary validation.
- Submitted by
- Balaji Sundararaman
- Lab
- Gene Yeo, UCSD
- Grant
- U54HG007005
- Download
- MBLI_RN108PW_001_UPF1.png
UPF1 (Homo sapiens)
compliant
- Caption
- Western blot following shRNA against UPF1 in HepG2 whole cell lysate using UPF1 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against UPF1. UPF1 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.
- Submitted by
- Xintao Wei
- Lab
- Brenton Graveley, UConn
- Grant
- U54HG007005
- Download
- UPF1-HEPG2.png
UPF1 (Homo sapiens)
compliant
- Caption
- Western blot following shRNA against UPF1 in K562 whole cell lysate using UPF1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against UPF1. UPF1 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.
- Submitted by
- Xintao Wei
- Lab
- Brenton Graveley, UConn
- Grant
- U54HG007005
- Download
- UPF1-K562.png