1. Antibodies
  2. Homo sapiens + Mus musculus
  3. HIST2H3A + HIST2H3D + HIST2H3C + Hist1h3d + Hist1h3b + Hist2h3c2 + Hist2h3c1 + Hist1h3e + Hist1h3f + Hist2h3b + Hist1h3c

ENCAB723WFC

Antibody against Homo sapiens H3K27me3, Mus musculus H3K27me3

Mus musculus
any cell type or tissue
characterized to standards
Homo sapiens
any cell type or tissue
characterized to standards
Status
released
Source (vendor)
Active Motif
Product ID
61017
Lot ID
14013006
Host
mouse
Clonality
monoclonal
Purification
Protein G
Isotype
IgG1
Antigen description
Histone H3 trimethyl Lys27 antibody was raised against a peptide containing trimethyl Lys27 of human Histone H3
Aliases
bing-ren:39.4
External resources

Characterizations

H3K27me3 (Mus musculus)
Method: dot blot assay
Attachment from submitter
Attachment from submitters
compliant
Caption
Performed dot blot analysis using Millipore’s AbSurance™ Complete Core Histone Antibody Specificity Array (cat# 16-668, http://www.millipore.com/catalogue/item/16-668#). The top panel contains histone H2A, H2B, and H4 peptides while the bottom panel has histone H3 peptides. The antibody recognized the H3K27me3 peptide 100% (noted with red #1).
Submitted by
Ah Young Lee
Lab
Bing Ren, UCSD
H3K27me3 (Homo sapiens)
Method: dot blot assay
Attachment from submitter
Attachment from submitters
compliant
Caption
Performed dot blot analysis using Millipore’s AbSurance™ Complete Core Histone Antibody Specificity Array (cat# 16-668, http://www.millipore.com/catalogue/item/16-668#). The top panel contains histone H2A, H2B, and H4 peptides while the bottom panel has histone H3 peptides. The antibody recognized the H3K27me3 peptide 100% (noted with red #1).
Submitted by
Cricket Sloan
Lab
Bing Ren, UCSD
H3K27me3 (Homo sapiens)
HCT116HEK293
Method: immunoblot
Attachment from submitter
Attachment from submitters
compliant
Caption
An immunoblot using whole cell extract of two human cell lines and one mouse ES cell line shows positive bands at the size of the histone (17kDa), while the recombinant unmodified version of that histone shows a lack of reactivity. The histone bands detected in the extracts constitute 100% of the protein signal and show at least 10-fold enrichment relative to any other band. This signal is also at least 10-fold enriched relative to that detected using the unmodified recombinant histone.
Submitted by
Ah Young Lee
Lab
Bing Ren, UCSD
H3K27me3 (Mus musculus)
TT2
Method: immunoblot
Attachment from submitter
Attachment from submitters
compliant
Caption
An immunoblot using whole cell extract of two human cell lines and one mouse ES cell line shows positive bands at the size of the histone (17kDa), while the recombinant unmodified version of that histone shows a lack of reactivity. The histone bands detected in the extracts constitute 100% of the protein signal and show at least 10-fold enrichment relative to any other band. This signal is also at least 10-fold enriched relative to that detected using the unmodified recombinant histone.
Submitted by
Ah Young Lee
Lab
Bing Ren, UCSD