ENCAB721ICQ

Antibody against Homo sapiens H3K27ac

Homo sapiens
any cell type or tissue
not characterized to standards
Status
released
Source (vendor)
Abcam
Product ID
ab4729
Lot ID
GR52206-1
Characterized targets
H3K27ac (Homo sapiens)
Host
rabbit
Clonality
polyclonal
Aliases
bradley-bernstein:PchAb 413
External resources

Characterizations

H3K27ac (Homo sapiens)
Method: dot blot assay
Attachment from submitter
not compliant
Submitted by
Kathrina Onate
Lab
Bradley Bernstein, Broad
H3K27ac (Homo sapiens)
K562HEK293
Method: immunoblot
Attachment from submitter
compliant
Caption
0.25ug unmodified Recombinant Histone H3 (Millipore 14-494) and 5*10^5 cells equivalent of HEK and K562 Nuclear Lysates were resolved by electrophoresis on a 4-12% acrylamide gel. After separation, the samples were transferred to a nitrocellulose membrane with an Invitrogen iBlot system. Membrane was blocked for an hour in room temperature, with 5% nonfat dry milk and blotted with primary antibody in the appropriate concentration over night at 4c. Membrane was washed and blotted with secondary HRP-conjugated antibody. Detection was made with Optiblot ECL Detect Kit (ab133406) for 2 min. Panel A: anti Histone H3 (Abcam ab1791), band of expected size (~17kDa) visualized in both unmodified recombinant histone H3 and NL, at similar intensities.Panel B: Band of expected size (~17kDa) visualized in NL, and cannot be detected in unmodified recombinant Histone H3. Antibody seems to be specific since no reactivity with nonhistone proteins or unmodified histones was detected
Submitted by
Noam Shoresh
Lab
Bradley Bernstein, Broad