1. Antibodies
  2. Homo sapiens
  3. MAFF

ENCAB717EYF

Alternate accession: ENCAB000AII

Antibody against Homo sapiens MAFF

Homo sapiens
K562, HeLa-S3, HepG2
characterized to standards
Homo sapiens
GM12878, any cell type or tissue
partially characterized
Status
released
Source (vendor)
Sigma
Product ID
M8194
Lot ID
125K4837
Characterized targets
MAFF (Homo sapiens)
Host
rabbit
Clonality
polyclonal
Antigen description
Raised against synthetic peptide corresponding to amino acids 147-163 of human MAFF. The immunizing peptide does not cross-react with other members of the family. Antibody Target: MAFF
Aliases
michael-snyder:AS-178

Characterizations

MAFF (Homo sapiens)
GM12878
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
not submitted for review by lab
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: GM12878 using the antibody M8194. The image shows western blot analysis of input, flowthrough, immunoprecipitate, and mock immunoprecipitate using IgG. Target molecular weight: 18.0.
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
MAFF (Homo sapiens)
HepG2
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
compliant
Caption
Immunoprecipitation of MAFF from HepG2 cells using M8194. Lane 1: input nuclear lysate. Lane 2: material immunoprecipitated with M8194. Lane 3: material immunoprecipitated using control IgG. Band A was excised from gel and subject to analysis by mass spectrometry.
Submitted by
Kathrina Onate
Lab
Michael Snyder, Stanford
MAFF (Homo sapiens)
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
not submitted for review by lab
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: HEK293T, using the antibody M8194. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 18.0
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
MAFF (Homo sapiens)
GM12878K562HeLa-S3HepG2
Method: immunoblot
Attachment from submitter
Attachment from submitters
compliant
Caption
Western blot analysis of nuclear lysates prepared from multiple cells lines loaded in the order: GM12878, K562, HeLaS3, HepG2 using the antibody M8194. Molecular Weight: 18.0
Reviewer comment
Marked band in GM12878 does not comprise of >50% of the total immunoreactive signal in the lane.
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
MAFF (Homo sapiens)
Method: immunoprecipitation followed by mass spectrometry
Attachment PDF Icon
compliant
Caption
IP followed by mass spectrometry: Briefly, protein was immunoprecipitated from HepG2 nuclear cell lysates using M8194, and the IP fraction was loaded on a 10% polyacrylamide gel (NuPAGE Bis-Tris Gel) and separated with an Invitrogen NuPAGE electrophoresis system. The gel was stained by Colloidial Coomassie G-250 stain, gel fragments corresponding to the bands indicated were excised. Then proteins were trypsinized using the in-gel digestion method. Digested proteins were analyzed on an Orbitrap Elite mass spectrometer (Thermo Scientific) by the nanoLC-ESI-MS/MS technique. Peptides were identified by the SEQUEST algorithm and filtered with a high confidence threshold (Peptide false discovery rate < 1%, 2 unique peptides per protein minimum, mass error < 10 ppm).
Submitted by
Kathrina Onate
Lab
Michael Snyder, Stanford