ENCAB707QIL

Antibody against Homo sapiens POLR2A

Homo sapiens
GM12878, HepG2, K562, endothelial cell of umbilical vein
partially characterized
Status
released
Source (vendor)
Abcam
Product ID
ab5131
Lot ID
GR37362-1
Characterized targets
POLR2A (Homo sapiens)
Host
rabbit
Clonality
polyclonal
Antigen description
S5p1 refers to the mono-phosphorylated serine found in the amino acid 5 position of the C-terminal domain (CTD) repeat YSPTSPS of RNA polymerase II
Aliases
bradley-bernstein:PchAb 162-V
External resources

Characterizations

POLR2A (Homo sapiens)
K562HepG2GM12878endothelial cell of umbilical vein
Method: immunoblot
Attachment from submitter
compliant
Caption
Nuclear lysates from K562 (10ug), HepG2 (10 ug), GM12878 (10ug), and HUVEC (10ug) were loaded into a 3-8% TA gel in 1X TArunning buffer Wet transfer, nitrocellulose, 30V for 1.5 hours, followed by Blocking with 5% BSA in TBS-T, 1 hour at RT. Antibodies dilulted in TBS-T with 0.5% BSA. 1 second exposure in ChemiDoc.
Submitted by
Noam Shoresh
Lab
Bradley Bernstein, Broad