1. Antibodies
  2. Homo sapiens
  3. SRSF3

ENCAB701PQV

Alternate accession: ENCAB655XAK

Antibody against Homo sapiens SRSF3

Homo sapiens
HepG2, K562
characterized to standards
Homo sapiens
any cell type or tissue
partially characterized
Status
released
Source (vendor)
MBLI
Product ID
RN080PW
Lot ID
001
Characterized targets
SRSF3 (Homo sapiens)
Host
rabbit
Clonality
polyclonal
Isotype
IgG
Aliases
xiang-dong-fu:SRSF3
External resources

Characterizations

SRSF3 (Homo sapiens)
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
not submitted for review by lab
Caption
IP-WB analysis of K562 whole cell lysate using SRSF3 specific antibody. Lane 1 is 2.5% of 0.5mg input lysate, lane 2 is 2.5% of supernatant after immunoprecipitation and Lane 3 is 50% of IP enrichment using rabbit polyclonal Anti-SRSF3(SRp20)pAb. This antibody did not meet our primary validation criteria using our standard IP protocol in the indicated cell type.
Submitted by
Balaji Sundararaman
Lab
Gene Yeo, UCSD
SRSF3 (Homo sapiens)
Method: knockdown or knockout
Attachment from submitter
Attachment from submitters
compliant
Caption
Western blot following shRNA against SRSF3 in HepG2 whole cell lysate using SRSF3 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against SRSF3.SRSF3 protein appears as the green band, Tubulin serves as a control and appears in red.
Submitted by
Xintao Wei
Lab
Brenton Graveley, UConn
SRSF3 (Homo sapiens)
Method: knockdown or knockout
Attachment from submitter
Attachment from submitters
compliant
Caption
Western blot following CRISPR against SRSF3 in K562 whole cell lysate using SRSF3 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against SRSF3.SRSF3 protein appears as the green band, Tubulin serves as a control and appears in red.
Submitted by
Xintao Wei
Lab
Brenton Graveley, UConn
SRSF3 (Homo sapiens)
HepG2
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
compliant
Caption
IP-Western Blot analysis of HepG2 whole cell lysate using SRSF3 specific antibody. Lane 1 is 3% of ten million whole cell lysate input (lane under '15% input') , lane 2 is 20% of IP enrichment using rabbit normal IgG (lane under 'IgG') and lane 3 is 20% IP enrichment using rabbit polyclonal anti-SRSF3 antibody (lanes under 'anti-SRSF3'). Asterisk indicates heavy chain of antibody.
Submitted by
Marcus Ho
Lab
Xiang-Dong Fu, UCSD
Download
 SRSF3_HepG2.png
SRSF3 (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
compliant
Caption
IP-Western Blot analysis of K562 whole cell lysate using SRSF3 specific antibody. Lane 1 is 3% of ten million whole cell lysate input (lane under '15% input') , lane 2 is 20% of IP enrichment using rabbit normal IgG (lane under 'IgG') and lane 3 is 20% IP enrichment using rabbit polyclonal anti-SRSF3 antibody (lanes under 'anti-SRSF3'). Asterisk indicates heavy chain of antibody.
Submitted by
Marcus Ho
Lab
Xiang-Dong Fu, UCSD
Download
 SRSF3_K562.png