ENCAB686PBA

Antibody against Homo sapiens ZC3H11A

Homo sapiens
K562, HepG2
characterized to standards
Homo sapiens
any cell type or tissue
partially characterized
Status
released
Source (vendor)
Bethyl Labs
Product ID
A301-524A
Lot ID
1
Characterized targets
ZC3H11A (Homo sapiens)
Host
rabbit
Clonality
polyclonal
Purification
affinity
Isotype
IgG
External resources

Characterizations

ZC3H11A (Homo sapiens)
Method: immunoprecipitation
Attachment from submitter
Caption
IP-WB analysis of K562 whole cell lysate using ZC3H11A specific antibody. Lane 1 is 2.5% of 0.5mg input lysate, lane 2 is 2.5% of supernatant after immunoprecipitation and Lane 3 is 50% of IP enrichment using rabbit polyclonal ZC3H11A Antibody. This antibody passes preliminary validation and will be further pursued for primary and secondary validation.
Submitted by
Balaji Sundararaman
Lab
Gene Yeo, UCSD
ZC3H11A (Homo sapiens)
HepG2
Method: immunoprecipitation
Attachment from submitter
compliant
Caption
Representative image of immunoprecipitation performed on whole cell extracts from the HepG2 cell line using the ZC3H11A-specific antibody A301-524A. Lane 1: Input from IP using control IgG. Lane 2: Immunoprecipitated material using control IgG. Lane 3: Input from IP using ZC3H11A antibody. Lane 4: Immunoprecipitated material using ZC3H11A antibody. Outlined regions were excised from gel and subjected to analysis by mass spectrometry. Target molecular weight: 89.13 kDa.
Submitted by
Steven Blue
Lab
Gene Yeo, UCSD
ZC3H11A (Homo sapiens)
HepG2
Method: immunoprecipitation
Attachment from submitter
compliant
Caption
IP-Western Blot analysis of HepG2 whole cell lysate using ZC3H11A specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-ZC3H11A antibody (lanes under 'ZC3H11A').
Submitted by
Steven Blue
Lab
Gene Yeo, UCSD
ZC3H11A (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
compliant
Caption
Representative image of immunoprecipitation performed on whole cell extracts from the K562 cell line using the ZC3H11A-specific antibody A301-524A. Lane 1: Input from IP using control IgG. Lane 2: Immunoprecipitated material using control IgG. Lane 3: Input from IP using ZC3H11A antibody. Lane 4: Immunoprecipitated material using ZC3H11A antibody. Outlined regions were excised from gel and subjected to analysis by mass spectrometry. Target molecular weight: 89.13 kDa.
Submitted by
Steven Blue
Lab
Gene Yeo, UCSD
ZC3H11A (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
compliant
Caption
IP-Western Blot analysis of K562 whole cell lysate using ZC3H11A specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-ZC3H11A antibody (lanes under 'ZC3H11A').
Submitted by
Steven Blue
Lab
Gene Yeo, UCSD
ZC3H11A (Homo sapiens)
Method: immunoprecipitation followed by mass spectrometry
compliant
Caption
IP followed by mass spectrometry. Protein was immunoprecipitated from HepG2 whole cell lysates using the antibody A301-524A, loaded on a 4-12% NuPAGE Bis-Tris gel, and separated via electrophoresis. Using a reference western blot done in parallel, gel pieces corresponding to the sections indicated were excised and submitted for analysis by the UCSD Biomolecular and Proteomics Mass Spectrometry Facility.
Submitted by
Steven Blue
Lab
Gene Yeo, UCSD
ZC3H11A (Homo sapiens)
Method: immunoprecipitation followed by mass spectrometry
compliant
Caption
IP followed by mass spectrometry. Protein was immunoprecipitated from K562 whole cell lysates using the antibody A301-524A, loaded on a 4-12% NuPAGE Bis-Tris gel, and separated via electrophoresis. Using a reference western blot done in parallel, gel pieces corresponding to the sections indicated were excised and submitted for analysis by the UCSD Biomolecular and Proteomics Mass Spectrometry Facility.
Submitted by
Steven Blue
Lab
Gene Yeo, UCSD
ZC3H11A (Homo sapiens)
Method: knockdown or knockout
Attachment from submitter
compliant
Caption
Western blot following CRISPR against ZC3H11A in HepG2 whole cell lysate using ZC3H11A specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against ZC3H11A.ZC3H11A protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.
Reviewer comment
The band is above 116 kDa and the expected size is 89 kDa, meaning the band is (116-89)/89=30.34% larger than the expected size. However, the vendor of ZC3H11A gels also shows the protein running at 116 kDa, hence it is compliant.
Submitted by
Xintao Wei
Lab
Brenton Graveley, UConn
ZC3H11A (Homo sapiens)
Method: knockdown or knockout
Attachment from submitter
compliant
Caption
Western blot following CRISPR against ZC3H11A in K562 whole cell lysate using ZC3H11A specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against ZC3H11A. ZC3H11A protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.
Submitted by
Xintao Wei
Lab
Brenton Graveley, UConn