Mus musculus
any cell type or tissue
characterized to standards
Homo sapiens
any cell type or tissue
Status
released
Source (vendor)
Cell Signaling
Product ID
9727
Lot ID
2
Host
rabbit
Clonality
polyclonal
Purification
affinity
Isotype
IgG
Antigen description
Synthetic peptide corresponding to the amino terminus of histone H3 in which lysine 4 is tri-methylated
Aliases
bing-ren:49.8
External resources

Characterizations

H3K4me3 (Mus musculus)
Method: dot blot assay
Attachment from submitter
compliant
Caption
Performed dot blot analysis using Millipore’s AbSurance™ Complete Core Histone Antibody Specificity Array (cat# 16-668, http://www.millipore.com/catalogue/item/16-668#). The top panel contains histone H2A, H2B, and H4 peptides while the bottom panel has histone H3 peptides. The antibody recognized the H3K4me3 peptide 100% (noted with red #1).
Submitted by
Ah Young Lee
Lab
Bing Ren, UCSD
H3K4me3 (Homo sapiens)
Method: dot blot assay
Attachment from submitter
compliant
Caption
Performed dot blot analysis using Millipore’s AbSurance™ Complete Core Histone Antibody Specificity Array (cat# 16-668, http://www.millipore.com/catalogue/item/16-668#). The top panel contains histone H2A, H2B, and H4 peptides while the bottom panel has histone H3 peptides. The antibody recognized the H3K4me3 peptide 100% (noted with red #1).
Submitted by
Ah Young Lee
Lab
Bing Ren, UCSD
H3K4me3 (Homo sapiens)
A375HEK293
Method: immunoblot
Attachment from submitter
compliant
Caption
An immunoblot using whole cell extract of two human cell lines and two mouse cell lines shows positive bands at the size of the histone (17kDa), while the recombinant unmodified version of that histone shows a lack of reactivity. The histone bands detected in the extracts constitute 100% of the protein signal and show at least 10-fold enrichment relative to any other band. This signal is also at least 10-fold enriched relative to that detected using the unmodified recombinant histone.
Submitted by
Ah Young Lee
Lab
Bing Ren, UCSD
H3K4me3 (Mus musculus)
embryonic fibroblastembryonic stem cell
Method: immunoblot
Attachment from submitter
compliant
Caption
An immunoblot using whole cell extract of two human cell lines and two mouse cell lines shows positive bands at the size of the histone (17kDa), while the recombinant unmodified version of that histone shows a lack of reactivity. The histone bands detected in the extracts constitute 100% of the protein signal and show at least 10-fold enrichment relative to any other band. This signal is also at least 10-fold enriched relative to that detected using the unmodified recombinant histone.
Submitted by
Ah Young Lee
Lab
Bing Ren, UCSD