ENCAB682XRE
Antibody against Homo sapiens H3K4me3, Mus musculus H3K4me3
Mus musculus
any cell type or tissue
characterized to standards
Homo sapiens
any cell type or tissue
characterized to standards
- Status
- released
- Source (vendor)
- Cell Signaling
- Product ID
- 9727
- Lot ID
- 2
- Characterized targets
- H3K4me3 (Homo sapiens), H3K4me3 (Mus musculus)
- Host
- rabbit
- Clonality
- polyclonal
- Purification
- affinity
- Isotype
- IgG
- Antigen description
- Synthetic peptide corresponding to the amino terminus of histone H3 in which lysine 4 is tri-methylated
- Aliases
- bing-ren:49.8
- External resources
Characterizations
H3K4me3 (Mus musculus)
compliant
- Caption
- Performed dot blot analysis using Millipore’s AbSurance™ Complete Core Histone Antibody Specificity Array (cat# 16-668, http://www.millipore.com/catalogue/item/16-668#). The top panel contains histone H2A, H2B, and H4 peptides while the bottom panel has histone H3 peptides. The antibody recognized the H3K4me3 peptide 100% (noted with red #1).
- Submitted by
- Ah Young Lee
- Lab
- Bing Ren, UCSD
- Grant
- U54HG006997
- Download
- bing-ren_49.8_dotblot.JPG
H3K4me3 (Homo sapiens)
compliant
- Caption
- Performed dot blot analysis using Millipore’s AbSurance™ Complete Core Histone Antibody Specificity Array (cat# 16-668, http://www.millipore.com/catalogue/item/16-668#). The top panel contains histone H2A, H2B, and H4 peptides while the bottom panel has histone H3 peptides. The antibody recognized the H3K4me3 peptide 100% (noted with red #1).
- Submitted by
- Ah Young Lee
- Lab
- Bing Ren, UCSD
- Grant
- U54HG006997
- Download
- bing-ren_49.8_dotblot.JPG
H3K4me3 (Homo sapiens)
A375HEK293
compliant
- Caption
- An immunoblot using whole cell extract of two human cell lines and two mouse cell lines shows positive bands at the size of the histone (17kDa), while the recombinant unmodified version of that histone shows a lack of reactivity. The histone bands detected in the extracts constitute 100% of the protein signal and show at least 10-fold enrichment relative to any other band. This signal is also at least 10-fold enriched relative to that detected using the unmodified recombinant histone.
- Submitted by
- Ah Young Lee
- Lab
- Bing Ren, UCSD
- Grant
- U54HG006997
- Download
- bing-ren_49.8_Western.JPG
H3K4me3 (Mus musculus)
embryonic fibroblastembryonic stem cell
compliant
- Caption
- An immunoblot using whole cell extract of two human cell lines and two mouse cell lines shows positive bands at the size of the histone (17kDa), while the recombinant unmodified version of that histone shows a lack of reactivity. The histone bands detected in the extracts constitute 100% of the protein signal and show at least 10-fold enrichment relative to any other band. This signal is also at least 10-fold enriched relative to that detected using the unmodified recombinant histone.
- Submitted by
- Ah Young Lee
- Lab
- Bing Ren, UCSD
- Grant
- U54HG006997
- Download
- bing-ren_49.8_Western.JPG