ENCAB681ELK
Antibody against Homo sapiens H3K9me2
Homo sapiens
any cell type or tissue
characterized to standards
- Status
- released
- Source (vendor)
- Cell Signaling
- Product ID
- 9753L
- Lot ID
- 1
- Characterized targets
- H3K9me2 (Homo sapiens)
- Host
- rabbit
- Clonality
- polyclonal
- Aliases
- bradley-bernstein:PchAb 121-V
- External resources
Characterizations
H3K9me2 (Homo sapiens)
K562
Method: immunoblot
compliant
- Caption
- 0.25ug unmodified Recombinant Histone H3 (Millipore 14-494) and 30ug of K562 Whole Cell Lysate (Abcam, ab7911) were resolved by electrophoresis on a 4-12% acrylamide gel. After separation, the samples were transferred to a nitrocellulose membrane with an Invitrogen iBlot system. Membrane was blocked for an hour in room temperature, with 5% nonfat dry milk and blotted with primary antibody in the appropriate concentration over night at 4c. Membrane was washed and blotted with secondary HRP-conjugated antibody. Detection was made with Optiblot ECL Detect Kit (ab133406) for 2 min. Panel A: anti Histone H3 (Abcam ab1791), band of expected size (~17kDa) visualized in both unmodified recombinant histone H3 and WCL, at similar intensities.Panel B: Band of expected size (~17kDa) visualized in WCL, and cannot be detected in unmodified recombinant Histone H3. Antibody seems to be specific since no reactivity with nonhistone proteins or unmodified histones was detected
- Submitted by
- Noam Shoresh
- Lab
- Bradley Bernstein, Broad
- Grant
- U54HG006991
H3K9me2 (Homo sapiens)
Method: dot blot assay
compliant
- Caption
- Performed dot blot analysis using a large set of modified peptides. The only peptide recognized by the H3K9me2 antibody was the correct one.
- Submitted by
- Noam Shoresh
- Lab
- Bradley Bernstein, Broad
- Grant
- U54HG006991