ENCAB679IZV
Antibody against Homo sapiens H3K9me3
Homo sapiens
any cell type or tissue
characterized to standards
- Status
- released
- Source (vendor)
- Abcam
- Product ID
- ab8898
- Lot ID
- GR3244172-1
- Characterized targets
- H3K9me3 (Homo sapiens)
- Host
- rabbit
- Clonality
- polyclonal
- Purification
- affinity
- Isotype
- IgG
Characterizations
H3K9me3 (Homo sapiens)
SU-DHL-2
Method: immunoprecipitation
compliant
- Caption
- Immunoprecipitation was performed on nuclear lysates prepared from SU-DHL-2 cells. The image shows western blot analysis of input, immunoprecipitate, and mock immunoprecipitate using IgG. Molecular Weight: 17kD.
- Submitter comment
- Signal in IP lane for molecular weights > 17kD can be explained as background which shows in the IgG lane (lane 3). Non-reactivity of this antibody lot against unmodified H3 is demonstrated on the dot blot.
- Submitted by
- Jessika Adrian
- Lab
- Michael Snyder, Stanford
- Grant
- UM1HG009442
- Download
- DHL2_H3K9me3_IP.jpg
H3K9me3 (Homo sapiens)
Method: dot blot assay
compliant
- Caption
- Performed dot blot analysis using Millipore’s AbSurance™ Complete Core Histone Antibody Specificity Array (cat# 16-667, http://www.millipore.com/catalogue/item/16-667#). The blot has histone H3 peptides. The antibody recognized the H3K9me3 peptide 100%, while the recombinant unmodified version of that histone shows a lack of reactivity.
- Submitted by
- Jessika Adrian
- Lab
- Michael Snyder, Stanford
- Grant
- UM1HG009442
- Download
- H3K9me3 dot blot.jpg
H3K9me3 (Homo sapiens)
MSLCL
Method: immunoblot
not submitted for review by lab
- Caption
- Western blot analysis of nuclear lysates prepared from LCL cells. Molecular Weight: 17kD.
- Submitter comment
- Target band at 17kD visible but gel shows signals of higher molecular weight.
- Submitted by
- Jessika Adrian
- Lab
- Michael Snyder, Stanford
- Grant
- UM1HG009442
- Download
- LCL_H3K9me3_WB.jpg