1. Antibodies
  2. Homo sapiens
  3. THRA

ENCAB669LID

Antibody against Homo sapiens THRA

Homo sapiens
K562
characterized to standards
Status
released
Source (vendor)
Origene
Product ID
TA805187
Lot ID
W001
Characterized targets
THRA (Homo sapiens)
Host
mouse
Clonality
monoclonal
Aliases
michael-snyder:AS-1348
External resources

Characterizations

THRA (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
not compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: K562, using the antibody TA805187. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 54.816
Reviewer comment
IgG shares the same band.
Submitted by
Denis Salins
Lab
Michael Snyder, Stanford
THRA (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line K562 using the antibody TA805187. Lane 1: input nuclear lysate. Lane 2: material immunoprecipitated with antibody. Lane 3: material immunoprecipitated using control IgG. Marked bands were excised from gel and subjected to analysis by mass spectrometry. Target molecular weight: 54.816.
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
THRA (Homo sapiens)
Method: immunoprecipitation followed by mass spectrometry
Attachment PDF Icon
compliant
Caption
IP followed by mass spectrometry. Briefly, protein was immunoprecipitated from K562 nuclear cell lysates using the antibody TA805187, and the IP fraction was loaded on a 10% polyacrylamide gel (NuPAGEBis-Tris Gel) and separated with an Invitrogen NuPAGE electrophoresis system. The gel was stained by ColloidialCoomassie G-250 stain, gel fragments corresponding to the bands indicated were excised. Then proteins were trypsinized using the in-gel digestion method. Digested proteins were analyzed on an Orbitrap Elite mass spectrometer (Thermo Scientific) by the nanoLC-ESI-MS/MS technique. Peptides were identified by the SEQUEST algorithm and filtered with a high confidence threshold (Peptide false discovery rate < 1%, 2 unique peptides per protein minimum, mass error < 10 ppm).
Submitter comment
None of the genes ranked higher or equally have been reported to bind DNA. Although EWSR1 has transcriptional activator function no sequence-specific DNA binding has been described.
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford