ENCAB657HDP

Antibody against Homo sapiens ZFX

Homo sapiens
RWPE1, HepG2, IMR-90, VCaP, C4-2B, HeLa-S3, K562, HCT116, MCF-7, GM12878, Panc1, RWPE2
characterized to standards
Homo sapiens
22Rv1, HEK293T
characterized to standards with exemption
Status
released
Source (vendor)
Cell Signaling
Product ID
CST_5419S
Lot ID
1
Characterized targets
ZFX (Homo sapiens)
Host
mouse
Clonality
monoclonal
Isotype
IgG2bκ
Antigen description
Monoclonal antibody is produced by immunizing animals with a recombinant fragment of human ZFX.
Aliases
michael-snyder:AS-1624
External resources

Characterizations

ZFX (Homo sapiens)
22Rv1HEK293TRWPE1RWPE2VCaP
Method: immunoblot
Attachment from submitter
compliant
Caption
Western blot analysis of nuclear lysates prepared from multiple cells lines loaded in the order: 22Rv1,HEK293T,RWPE-1,RWPE-2,VCaP using the antibody CST_5419S. Molecular Weight: 135.
Reviewer comment
Doublet in 22Rv1 and HEK293T, different from all other tested cell lines but marked band is consistent.
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
ZFX (Homo sapiens)
Method: knockdown or knockout
Attachment from submitter
compliant
Caption
Western blot following siRNA against ZFX in C42B cell lysate using ZFX specific antibody. Lane 1, 5 are C42B non-targeting control knockdown; lane 2, 6 are unmodified C42B cells; lane 3, 4, 7, 8 are two different siRNAs against ZFX. siRNA catalog information: Dharmacon L-006572-00-0005 siRNA sequence information: CCAAGUAGUAGUUGUUUAA, UGAAAUCGCUGACGAAGUU, GAAUGACCAUGGACACAGA, GCAACAUGCUAGUUACUUU
Submitted by
Jessika Adrian
Lab
Michael Snyder, Stanford
ZFX (Homo sapiens)
C4-2BGM12878HCT116HeLa-S3HepG2IMR-90K562MCF-7Panc1
Method: immunoblot
Attachment from submitter
compliant
Caption
Western blot analysis of nuclear lysates prepared from multiple cells lines loaded in the order: C4-2B,GM12878,HCT116,HeLa-S3,HepG2,IMR90,K562,MCF-7,Panc1 using the antibody CST_5419S. Molecular Weight: 135.
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford