ENCAB653SNR
Antibody against Homo sapiens SRSF4
Homo sapiens
K562, HepG2
characterized to standards
Homo sapiens
any cell type or tissue
partially characterized
- Status
- released
- Source (vendor)
- Bethyl Labs
- Product ID
- A303-670A
- Lot ID
- 1
- Characterized targets
- SRSF4 (Homo sapiens)
- Host
- rabbit
- Clonality
- polyclonal
- Purification
- affinity
- Isotype
- IgG
- Aliases
- xiang-dong-fu:SRSF4
- External resources
Characterizations
SRSF4 (Homo sapiens)
Method: immunoprecipitation
not submitted for review by lab
- Caption
- IP-WB analysis of K562 whole cell lysate using SRSF4 specific antibody. Lane 1 is 2.5% of 0.5mg input lysate, lane 2 is 2.5% of supernatant after immunoprecipitation and Lane 3 is 50% of IP enrichment using rabbit polyclonal Rabbitanti-SRp75Antibody. This antibody passes preliminary validation and will be further pursued for primary and secondary validation.
- Submitted by
- Balaji Sundararaman
- Lab
- Gene Yeo, UCSD
- Grant
- U54HG007005
- Download
- Bethyl_A303-670A_1_SRSF4.png
SRSF4 (Homo sapiens)
HepG2
Method: immunoprecipitation
compliant
- Caption
- IP-Western Blot analysis of HepG2 whole cell lysate using SRSF4 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-SRSF4 antibody (lanes under 'SRSF4').
- Reviewer comment
- Multiple bands where marked. Could be rescued with a knockdown if it can be shown all bands correspond to the factor of interest.
- Submitted by
- Steven Blue
- Lab
- Gene Yeo, UCSD
- Grant
- U54HG007005
- Download
- HepG2_Bethyl_A303-670A_1_SRSF4.png
SRSF4 (Homo sapiens)
K562
Method: immunoprecipitation
compliant
- Caption
- IP-Western Blot analysis of K562 whole cell lysate using SRSF4 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input; lane 2 is 10% of IP enrichment using rabbit normal IgG (lanes under 'lgG'); Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-SRSF4 antibody (lanes under 'SRSF4').
- Submitted by
- Xintao Wei
- Lab
- Gene Yeo, UCSD
- Grant
- U54HG007005
- Download
- K562_Bethyl_A303-670A_1_SRSF4.png
SRSF4 (Homo sapiens)
Method: knockdown or knockout
compliant
- Caption
- Western blot following CRISPR against SRSF4 in K562 whole cell lysate using SRSF4 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against SRSF4.SRSF4 protein appears as the green band, Tubulin serves as a control and appears in red.
- Reviewer comment
- Knock-down looks good relative to the control, though running at higher than expected size.
- Submitted by
- Xintao Wei
- Lab
- Brenton Graveley, UConn
- Grant
- U54HG007005
- Download
- SRSF4-K562.png