1. Antibodies
  2. Homo sapiens
  3. FMR1

ENCAB650WPE

Antibody against Homo sapiens FMR1

Homo sapiens
K562
characterized to standards
Status
released
Source (vendor)
Thermo Fisher
Product ID
PA5-34584
Lot ID
TK2671134
Characterized targets
FMR1 (Homo sapiens)
Host
rabbit
Clonality
polyclonal
Purification
affinity
Isotype
IgG
Antigen description
Synthetic peptide conjugated to KLH derived from within residues 550 to the C-terminus of Human FMRP.

Characterizations

FMR1 (Homo sapiens)
Method: knockdown or knockout
Attachment from submitter
Attachment from submitters
compliant
Caption
Western blot following shRNA against FMR1 in HepG2 whole cell lysate using FMR1 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different shRNA against FMR1. FMR1 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
Reviewer comment
Issue 2: Presence of multiple bands reacting with the AB. The DCC is wondering if identification of two isoforms is not a problem and could be regarded as compliant if both isoforms are going down upon knockdown? Mendenhall Response: Assuming, the siRNA targets both isoforms, this is not a problem, but would be nice if they have other support for two isoforms they can note. Other westerns or known splicing/isoforms in cell type of interest?
Submitted by
Xintao Wei
Lab
Brenton Graveley, UConn
FMR1 (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
compliant
Caption
IP-WB analysis of K562 whole cell lysate using the FMR1 specific antibody, PA5-34584. Lanes 1 and 3 are 2.5% of five million whole cell lysate input. Lanes 2 and 4 are 50% of IP enrichment from five million whole cell lysate using normal IgG antibody and the FMR1-specific antibody, PA5-34584. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
Submitted by
Steven Blue
Lab
Gene Yeo, UCSD