ENCAB649LYF
Alternate accession: ENCAB000AHH
Antibody against Homo sapiens HDAC1
Homo sapiens
HepG2, endothelial cell of umbilical vein
characterized to standards
Homo sapiens
any cell type or tissue
partially characterized
- Status
- released
- Source (vendor)
- Santa Cruz Biotech
- Product ID
- sc-6298
- Lot ID
- H0509
- Characterized targets
- HDAC1 (Homo sapiens)
- Host
- goat
- Clonality
- polyclonal
- Antigen description
- histone deacetylase
- Aliases
- bradley-bernstein:PchAb 134-V
- External resources
Characterizations
HDAC1 (Homo sapiens)
compliant
- Caption
- This validation relies on the use of antibodies to different members of a known complex, in HepG2 cells, and the demonstration that highly similar patterns of enrichment are obtained with each antibody. The second track shown used ENCAB000AKO, an antibody to SAP30 (PchAb 164-V), another member of the Sin3a complex. Overall correlation score: HDAC1 vs SAP30: 0.9175
- Submitted by
- Kathrina Onate
- Lab
- Bradley Bernstein, Broad
- Grant
- U54HG006991
- Download
- HDAC1 PchAb 134-V SAV.pdf
HDAC1 (Homo sapiens)
compliant
- Caption
- This validation relies on the use of antibodies to different members of a known complex, in HepG2 cells, and the demonstration that highly similar patterns of enrichment are obtained with each antibody. The second track shown used an antibody to SAP30 (PchAb 164-V), another member of the Sin3a complex. Overall correlation score: 0.9175
- Submitter comment
- SAV8
- Submitted by
- Nina Farrell
- Lab
- Bradley Bernstein, Broad
- Grant
- U54HG006991
- Download
- HDAC1 PchAb 134-V SAV.pdf
HDAC1 (Homo sapiens)
HepG2endothelial cell of umbilical vein
compliant
- Caption
- Nuclear lysates from HepG2 (10ug), HUVEC (10ug), were loaded into a 4-12% Bis-Tris gel in 1X MES running buffer. After separation, the samples were transferred to a nitrocellulose membrane using the iblot system. Membrane was blocked for an hour in room temperature, with 5% BSA in TBS-T and blotted with primary antibody in the appropriate concentration over night at 4c. Membrane was washed and blotted with secondary HRP-conjugated antibody. Detection was made with Optiblot ECL Detect Kit (ab133406) for 2 min. a single band of the expected size was detected (~60kDa).
- Submitted by
- Noam Shoresh
- Lab
- Bradley Bernstein, Broad
- Grant
- U54HG006991
HDAC1 (Homo sapiens)
not reviewed
- Caption
- Antibody was validated by use of correlation analysis of ChIP-String and ChIP-Seq data
- Submitted by
- Bradley Bernstein
- Lab
- Bradley Bernstein, Broad
- Grant
- U54HG004570
HDAC1 (Homo sapiens)
not reviewed
- Submitted by
- Bradley Bernstein
- Lab
- Bradley Bernstein, Broad
- Grant
- U54HG004570