ENCAB649LYF

Alternate accession: ENCAB000AHH

Antibody against Homo sapiens HDAC1

Homo sapiens
HepG2, endothelial cell of umbilical vein
characterized to standards
Homo sapiens
any cell type or tissue
partially characterized
Status
released
Source (vendor)
Santa Cruz Biotech
Product ID
sc-6298
Lot ID
H0509
Host
goat
Clonality
polyclonal
Antigen description
histone deacetylase
Aliases
bradley-bernstein:PchAb 134-V

Characterizations

HDAC1 (Homo sapiens)
Method: ChIP-seq comparison
compliant
Caption
This validation relies on the use of antibodies to different members of a known complex, in HepG2 cells, and the demonstration that highly similar patterns of enrichment are obtained with each antibody. The second track shown used ENCAB000AKO, an antibody to SAP30 (PchAb 164-V), another member of the Sin3a complex. Overall correlation score: HDAC1 vs SAP30: 0.9175
Submitted by
Kathrina Onate
Lab
Bradley Bernstein, Broad
HDAC1 (Homo sapiens)
Method: ChIP-seq comparison
compliant
Caption
This validation relies on the use of antibodies to different members of a known complex, in HepG2 cells, and the demonstration that highly similar patterns of enrichment are obtained with each antibody. The second track shown used an antibody to SAP30 (PchAb 164-V), another member of the Sin3a complex. Overall correlation score: 0.9175
Submitter comment
SAV8
Submitted by
Nina Farrell
Lab
Bradley Bernstein, Broad
HDAC1 (Homo sapiens)
HepG2endothelial cell of umbilical vein
Method: immunoblot
Attachment from submitter
compliant
Caption
Nuclear lysates from HepG2 (10ug), HUVEC (10ug), were loaded into a 4-12% Bis-Tris gel in 1X MES running buffer. After separation, the samples were transferred to a nitrocellulose membrane using the iblot system. Membrane was blocked for an hour in room temperature, with 5% BSA in TBS-T and blotted with primary antibody in the appropriate concentration over night at 4c. Membrane was washed and blotted with secondary HRP-conjugated antibody. Detection was made with Optiblot ECL Detect Kit (ab133406) for 2 min. a single band of the expected size was detected (~60kDa).
Submitted by
Noam Shoresh
Lab
Bradley Bernstein, Broad
HDAC1 (Homo sapiens)
Method: ChIP-string comparison
not reviewed
Caption
Antibody was validated by use of correlation analysis of ChIP-String and ChIP-Seq data
Submitted by
Bradley Bernstein
Lab
Bradley Bernstein, Broad
HDAC1 (Homo sapiens)
Method: immunoblot
not reviewed
Submitted by
Bradley Bernstein
Lab
Bradley Bernstein, Broad