ENCAB644NSA

Antibody against Homo sapiens SMARCB1

Homo sapiens
GM12878, K562
partially characterized
Status
released
Source (vendor)
Bethyl Labs
Product ID
A301-087A
Lot ID
1
Characterized targets
SMARCB1 (Homo sapiens)
Host
rabbit
Clonality
polyclonal
Aliases
bradley-bernstein:PchAb 1297
External resources

Characterizations

SMARCB1 (Homo sapiens)
K562GM12878
Method: immunoblot
Attachment from submitter
compliant
Caption
Nuclear lysates from K562 (10ug) and GM12878 (10ug) were loaded into a 3-8% Tris-Acetate gel in 1X TA running buffer. After separation, the samples were transferred to a nitrocellulose membrane using iblot. Membrane was blocked for an hour in room temperature, with 5% BSA in TBS-T and blotted with primary antibody in the appropriate concentration over night at 4c. Membrane was washed and blotted with secondary HRP-conjugated antibody. Detection was made with Optiblot ECL Detect Kit (ab133406) for 2 min. Bands were detected at the expected size. (44 kDa)
Submitted by
Noam Shoresh
Lab
Bradley Bernstein, Broad