ENCAB644NSA
Antibody against Homo sapiens SMARCB1
Homo sapiens
K562, GM12878
partially characterized
- Status
- released
- Source (vendor)
- Bethyl Labs
- Product ID
- A301-087A
- Lot ID
- 1
- Characterized targets
- SMARCB1 (Homo sapiens)
- Host
- rabbit
- Clonality
- polyclonal
- Aliases
- bradley-bernstein:PchAb 1297
- External resources
Characterizations
SMARCB1 (Homo sapiens)
K562GM12878
Method: immunoblot
compliant
- Caption
- Nuclear lysates from K562 (10ug) and GM12878 (10ug) were loaded into a 3-8% Tris-Acetate gel in 1X TA running buffer. After separation, the samples were transferred to a nitrocellulose membrane using iblot. Membrane was blocked for an hour in room temperature, with 5% BSA in TBS-T and blotted with primary antibody in the appropriate concentration over night at 4c. Membrane was washed and blotted with secondary HRP-conjugated antibody. Detection was made with Optiblot ECL Detect Kit (ab133406) for 2 min. Bands were detected at the expected size. (44 kDa)
- Submitted by
- Noam Shoresh
- Lab
- Bradley Bernstein, Broad
- Grant
- U54HG006991
- Download
- SMARCB1_SNF5_Bethyl_A301-087A-1.png