ENCAB626JLZ

Homo sapiens

HepG2

characterized to standards

Homo sapiens

any cell type or tissue

partially characterized

Method: immunoprecipitation

not submitted for review by lab

Caption: IP-WB analysis of K562 whole cell lysate using EIF3D specific antibody. Lane 1 is 2.5% of 0.5mg input lysate, lane 2 is 2.5% of supernatant after immunoprecipitation and Lane 3 is 50% of IP enrichment using rabbit polyclonal eIF3D/EIF3S7 Antibody. This antibody passes preliminary validation and will be further pursued for primary and secondary validation.
Submitted by: Balaji Sundararaman
Lab: Gene Yeo, UCSD
Grant: U54HG007005
Download: Bethyl_A301-758A_1_EIF3D.png

Method: knockdown or knockout

compliant

Caption: Western blot following shRNA against EIF3D in K562 and HepG2 whole cell lysate using EIF3D specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 2 and 3 are two different shRNAs against EIF3D. Lanes 5-7 follow the same pattern, but in HepG2. EIF3D protein appears as the green band, GAPDH serves as a control and appears in red.
Submitted by: Brenton Graveley
Lab: Brenton Graveley, UConn
Grant: U54HG007005
Download: EIF3D_Secondary_Western.png
Documents: encode:Antibody_Characterization_ENCODE3_February2014.pdf

HepG2

Method: immunoprecipitation

compliant

Caption: IP-Western Blot analysis of HepG2 whole cell lysate using EIF3D specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-EIF3D antibody (lanes under 'EIF3D').
Submitted by: Steven Blue
Lab: Gene Yeo, UCSD
Grant: U54HG007005
Download: HepG2_Bethyl_A301-758A_1_EIF3D.png
Documents: encode:Antibody_Characterization_ENCODE3_August2015.pdf