1. Antibodies
  2. Homo sapiens
  3. KAT2B

ENCAB611ELJ

Antibody against Homo sapiens KAT2B

Homo sapiens
K562, A549
characterized to standards
Homo sapiens
HEK293T
partially characterized
Status
released
Source (vendor)
Abcam
Product ID
ab12188
Lot ID
GR60383
Characterized targets
KAT2B (Homo sapiens)
Host
rabbit
Aliases
michael-snyder:KAT2B-human_ab12188_GR60383
External resources

Characterizations

KAT2B (Homo sapiens)
A549
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: A549, using the antibody ab12188. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 93.013
Reviewer comment
The major band is just within lower bound of the allowed 20% size deviation.
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
KAT2B (Homo sapiens)
HEK293T
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
not submitted for review by lab
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: HEK293T using the antibody ab12188. The image shows western blot analysis of input, flowthrough, immunoprecipitate, and mock immunoprecipitate using IgG. Target molecular weight: 93.013.
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
KAT2B (Homo sapiens)
Method: immunoprecipitation followed by mass spectrometry
Attachment PDF Icon
compliant
Caption
IP followed by mass spectrometry: Briefly, protein was immunoprecipitated from K562 whole cell lysates using ab12188, and the IP fraction was loaded on a 10% polyacrylamide gel (NuPAGE Bis-Tris Gel) and separated with an Invitrogen NuPAGE electrophoresis system. The gel was silver-stained, gel fragments corresponding to the bands indicated were excised and destained using the SilverSNAP Stain for Mass Spectrometry (Pierce). Then proteins were treated with trypsin using the in-gel digestion method. Digested proteins were analyzed on a LTQ-Orbitrap (Thermo Scientific) b the nanoLC-ESI-MS/MS technique. Peptides were identified by the SEQUEST algorithm and filtered with a high confidence threshold (protein false discovery rate <1%, 2 peptides per protein minimum).
Submitted by
Kathrina Onate
Lab
Michael Snyder, Stanford
KAT2B (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
compliant
Caption
Immunoprecipitation of KAT2b/PCAF from K562 cells using ab12188. Lane 1: input nuclear lysate. Lane 2: material immunoprecipitated with ab12188. Lane 3: material immunoprecipitated using control IgG. Band A was excised from gel and subject to analysis by mass spectrometry. Expected size: ~93kD
Submitted by
Kathrina Onate
Lab
Michael Snyder, Stanford
Download
 PCAF_IP.png