ENCAB605GOL
Antibody against Homo sapiens ATF2
Homo sapiens
K562, GM12878, HepG2, MCF-7
characterized to standards
Homo sapiens
any cell type or tissue
partially characterized
- Status
- released
- Source (vendor)
- Bethyl Labs
- Product ID
- A301-649A
- Lot ID
- 1
- Characterized targets
- ATF2 (Homo sapiens)
- Host
- rabbit
- Clonality
- polyclonal
- Purification
- affinity
- Aliases
- michael-snyder:AS-886
- External resources
Characterizations
ATF2 (Homo sapiens)
GM12878
Method: immunoprecipitation
compliant
- Caption
- Immunoprecipitation was performed on nuclear extracts from the cell line: GM12878, using the antibody A301-649A. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 54.537
- Submitted by
- Denis Salins
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG006996
- Download
- 1064_1_ATF2_A301-649A_GM12878.jpg
ATF2 (Homo sapiens)
HepG2
Method: immunoprecipitation
compliant
- Caption
- Immunoprecipitation was performed on nuclear extracts from the cell line: HepG2, using the antibody A301-649A. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 54.537
- Submitted by
- Denis Salins
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG006996
- Download
- 1064_2_ATF2_A301-649A_HepG2.jpg
ATF2 (Homo sapiens)
MCF-7
Method: immunoprecipitation
compliant
- Caption
- Immunoprecipitation was performed on nuclear extracts from the cell line: MCF-7, using the antibody A301-649A. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 54.537
- Submitted by
- Denis Salins
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG006996
- Download
- 1064_3_ATF2_A301-649A_MCF7.jpg
ATF2 (Homo sapiens)
K562
Method: immunoprecipitation
compliant
- Caption
- Immunoprecipitation was performed on nuclear extracts from the cell line: K562, using the antibody A301-649A. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.
- Submitted by
- Denis Salins
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG006996
ATF2 (Homo sapiens)
Method: motif enrichment
compliant
- Caption
- The motif for target ATF2 is represented by the attached position weight matrix (PWM) derived from ENCFF079COS. Motif enrichment analysis was done by Dr. Zhizhuo Zhang (Broad Institute, Kellis Lab) using known motifs (http://compbio.mit.edu/encode-motifs/) and previously published ChIP-seq data (http://www.broadinstitute.org/~zzhang/motifpipeline/data/TrainSetInfo.txt). The accept probability score of the given transcription factor was calculated using a Bayesian approach. This analysis also includes three motif enrichment scores, computed by overlapping the motif instances with the given ChIP-seq peak locations, as well as an enrichment rank. For more information on the underlying statistical methods, please see the attached document. Accept probability score: 0.679797265 Global enrichment Z-score: 11.42001883 Positional bias Z-score: 14.13505792 Peak rank bias Z-score: 11.2755059 Enrichment rank: 4.0
- Submitted by
- Kathrina Onate
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG006996
ATF2 (Homo sapiens)
Method: motif enrichment
compliant
- Caption
- The motif for target ATF2 is represented by the attached position weight matrix (PWM) derived from ENCFF510RPN. Motif enrichment analysis was done by Dr. Zhizhuo Zhang (Broad Institute, Kellis Lab). Accept probability score: 0.675578605 Global enrichment Z-score: 11.31826966 Positional bias Z-score: 13.77468201 Peak rank bias Z-score: 9.959224405 Enrichment rank: 5.0
- Submitted by
- Kathrina Onate
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG006996
ATF2 (Homo sapiens)
Method: motif enrichment
compliant
- Caption
- The motif for target ATF2 is represented by the attached position weight matrix (PWM) derived from ENCFF896CPF. Motif enrichment analysis was done by Dr. Zhizhuo Zhang (Broad Institute, Kellis Lab). Accept probability score: 0.675578605 Global enrichment Z-score: 10.45358704 Peak rank bias Z-score: 8.536185812 Enrichment rank: 5.0
- Submitted by
- Kathrina Onate
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG006996
ATF2 (Homo sapiens)
Method: immunoprecipitation
not submitted for review by lab
- Caption
- Immunoprecipitation was performed on nuclear extracts from the cell line: HEK293T, using the antibody A301-649A. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 54.537
- Submitted by
- Nathaniel Watson
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG006996
- Download
- Expt1118_2-ATF2-A301-649A.JPG