ENCAB605GOL

Antibody against Homo sapiens ATF2

Homo sapiens
K562, GM12878, HepG2, MCF-7
characterized to standards
Homo sapiens
any cell type or tissue
partially characterized
Status
released
Source (vendor)
Bethyl Labs
Product ID
A301-649A
Lot ID
1
Characterized targets
ATF2 (Homo sapiens)
Host
rabbit
Clonality
polyclonal
Purification
affinity
Aliases
michael-snyder:AS-886
External resources

Characterizations

ATF2 (Homo sapiens)
GM12878
Method: immunoprecipitation
Attachment from submitter
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: GM12878, using the antibody A301-649A. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 54.537
Submitted by
Denis Salins
Lab
Michael Snyder, Stanford
ATF2 (Homo sapiens)
HepG2
Method: immunoprecipitation
Attachment from submitter
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: HepG2, using the antibody A301-649A. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 54.537
Submitted by
Denis Salins
Lab
Michael Snyder, Stanford
ATF2 (Homo sapiens)
MCF-7
Method: immunoprecipitation
Attachment from submitter
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: MCF-7, using the antibody A301-649A. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 54.537
Submitted by
Denis Salins
Lab
Michael Snyder, Stanford
ATF2 (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: K562, using the antibody A301-649A. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.
Submitted by
Denis Salins
Lab
Michael Snyder, Stanford
ATF2 (Homo sapiens)
Method: motif enrichment
compliant
Caption
The motif for target ATF2 is represented by the attached position weight matrix (PWM) derived from ENCFF079COS. Motif enrichment analysis was done by Dr. Zhizhuo Zhang (Broad Institute, Kellis Lab) using known motifs (http://compbio.mit.edu/encode-motifs/) and previously published ChIP-seq data (http://www.broadinstitute.org/~zzhang/motifpipeline/data/TrainSetInfo.txt). The accept probability score of the given transcription factor was calculated using a Bayesian approach. This analysis also includes three motif enrichment scores, computed by overlapping the motif instances with the given ChIP-seq peak locations, as well as an enrichment rank. For more information on the underlying statistical methods, please see the attached document. Accept probability score: 0.679797265 Global enrichment Z-score: 11.42001883 Positional bias Z-score: 14.13505792 Peak rank bias Z-score: 11.2755059 Enrichment rank: 4.0
Submitted by
Kathrina Onate
Lab
Michael Snyder, Stanford
ATF2 (Homo sapiens)
Method: motif enrichment
compliant
Caption
The motif for target ATF2 is represented by the attached position weight matrix (PWM) derived from ENCFF510RPN. Motif enrichment analysis was done by Dr. Zhizhuo Zhang (Broad Institute, Kellis Lab). Accept probability score: 0.675578605 Global enrichment Z-score: 11.31826966 Positional bias Z-score: 13.77468201 Peak rank bias Z-score: 9.959224405 Enrichment rank: 5.0
Submitted by
Kathrina Onate
Lab
Michael Snyder, Stanford
ATF2 (Homo sapiens)
Method: motif enrichment
compliant
Caption
The motif for target ATF2 is represented by the attached position weight matrix (PWM) derived from ENCFF896CPF. Motif enrichment analysis was done by Dr. Zhizhuo Zhang (Broad Institute, Kellis Lab). Accept probability score: 0.675578605 Global enrichment Z-score: 10.45358704 Peak rank bias Z-score: 8.536185812 Enrichment rank: 5.0
Submitted by
Kathrina Onate
Lab
Michael Snyder, Stanford
ATF2 (Homo sapiens)
Method: immunoprecipitation
Attachment from submitter
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: HEK293T, using the antibody A301-649A. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 54.537
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford