ENCAB603WLW

Antibody against Homo sapiens HNRNPA1

Homo sapiens
HepG2, K562
characterized to standards
Status
released
Source (vendor)
Santa Cruz Biotech
Product ID
sc-32301
Lot ID
I2413
Characterized targets
HNRNPA1 (Homo sapiens)
Host
mouse
Clonality
monoclonal
Purification
affinity
Isotype
IgG1
Antigen description
hnRNP A1 (4B10) is a mouse monoclonal antibody raised against full length partially purified hnRNP A1
External resources

Characterizations

HNRNPA1 (Homo sapiens)
HepG2
Method: immunoprecipitation
Attachment from submitter
compliant
Caption
IP-Western Blot analysis of HepG2 whole cell lysate using HNRNPA1 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 10% of IP enrichment using mouse normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using mouse monoclonal anti-HNRNPA1 antibody (lanes under 'HNRNPA1').
Submitted by
Balaji Sundararaman
Lab
Gene Yeo, UCSD
HNRNPA1 (Homo sapiens)
Method: knockdown or knockout
Attachment from submitter
compliant
Caption
Western blot following shRNA against HNRNPA1 in K562 and HepG2 whole cell lysate using HNRNPA1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against HNRNPA1. Lanes 5-8 follow the same pattern, but in HepG2. HNRNPA1 protein appears as the green band, Tubulin serves as a control and appears in red.
Submitted by
Xintao Wei
Lab
Brenton Graveley, UConn
HNRNPA1 (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
compliant
Caption
IP-Western Blot analysis of K562 whole cell lysate using HNRNPA1 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 10% of IP enrichment using mouse normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using mouse monoclonal anti-HNRNPA1 antibody (lanes under 'HNRNPA1').
Submitted by
Balaji Sundararaman
Lab
Gene Yeo, UCSD