ENCAB597TIV

Antibody against Homo sapiens IGF2BP2

Homo sapiens
K562, HepG2
characterized to standards
Status
released
Source (vendor)
MBLI
Product ID
RN008P
Lot ID
002
Characterized targets
IGF2BP2 (Homo sapiens)
Host
rabbit
Clonality
polyclonal
Purification
affinity
Antigen description
KLH-conjugated synthetic peptide KQQEQKYPQGVASQRSK ( 583-599 aa)
Antigen sequence
KQQEQKYPQGVASQRSK
External resources

Characterizations

IGF2BP2 (Homo sapiens)
HepG2
Method: immunoprecipitation
Attachment from submitter
compliant
Caption
IP-Western Blot analysis of HepG2 whole cell lysate using IGF2BP2 specific antibody. Lane 1 is 2.5% of five million whole cell lysate input, lane 2 is 2.5% of supernatant after immunoprecipitation and Lane 3 is 50% of IP enrichment using either rabbit polyclonal anti-IGF2BP2 antibody (lanes under 'IGF2BP2') or using rabbit normal IgG (lanes under 'IgG'). Stars indicate light and heavy chains of IgG background
Submitted by
Balaji Sundararaman
Lab
Gene Yeo, UCSD
IGF2BP2 (Homo sapiens)
Method: knockdown or knockout
Attachment from submitter
compliant
Caption
Western blot following shRNA against IGF2BP2 in K562 and HepG2 whole cell lysate using IGF2BP2 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against IGF2BP2. Lanes 5-7 follow the same pattern, but in HepG2. IGF2BP2 protein appears as the green band, Tubulin serves as a control and appears in red.
Submitted by
Xintao Wei
Lab
Brenton Graveley, UConn
IGF2BP2 (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
compliant
Caption
IP-Western Blot analysis of K562 whole cell lysate using IGF2BP2 specific antibody. Lane 1 is 2.5% of five million whole cell lysate input, lane 2 is 2.5% of supernatant after immunoprecipitation and Lane 3 is 50% of IP enrichment using either rabbit polyclonal anti-IGF2BP2 antibody (lanes under 'IGF2BP2') or using rabbit normal IgG (lanes under 'IgG'). Stars indicate light and heavy chains of IgG background
Submitted by
Balaji Sundararaman
Lab
Gene Yeo, UCSD