ENCAB593IKV

Antibody against Homo sapiens LARP7

Homo sapiens
K562
characterized to standards with exemption
Status
released
Source (vendor)
Bethyl Labs
Product ID
A303-724A
Lot ID
1
Characterized targets
LARP7 (Homo sapiens)
Host
rabbit
Clonality
polyclonal
Purification
affinity
Aliases
michael-snyder:AS-1422
External resources

Characterizations

LARP7 (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: K562, using the antibody A303-724A. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 66.899
Submitted by
Denis Salins
Lab
Michael Snyder, Stanford
LARP7 (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line K562 using the antibody A303-724A. Lane 1: input nuclear lysate. Lane 2: material immunoprecipitated with antibody. Lane 3: material immunoprecipitated using control IgG. Marked bands were excised from gel and subjected to analysis by mass spectrometry. Target molecular weight: 66.899.
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
LARP7 (Homo sapiens)
Method: immunoprecipitation followed by mass spectrometry
exempt from standards
Caption
IP followed by mass spectrometry. Briefly, protein was immunoprecipitated from K562 nuclear cell lysates using the antibody A303-724A, and the IP fraction was loaded on a 10% polyacrylamide gel (NuPAGEBis-Tris Gel) and separated with an Invitrogen NuPAGE electrophoresis system. The gel was stained by ColloidialCoomassie G-250 stain, gel fragments corresponding to the bands indicated were excised. Then proteins were trypsinized using the in-gel digestion method. Digested proteins were analyzed on an Orbitrap Elite mass spectrometer (Thermo Scientific) by the nanoLC-ESI-MS/MS technique. Peptides were identified by the SEQUEST algorithm and filtered with a high confidence threshold (Peptide false discovery rate < 1%, 2 unique peptides per protein minimum, mass error < 10 ppm).
Submitter comment
MCM2 is not sequence-specific DNA binding TF.
Reviewer comment
The ENCODE Antibody Review panel decided to exempt this characterization on 12/13/16 on the grounds that although MCM2 is ranked higher than LARP7 in the MS results as MCM2-7 are members of the Pre-RC (doi:10.1038/nrg2143) and should not be treated as sequence specific TFs.
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford