ENCAB592TEY

Antibody against Homo sapiens RBFOX2

Homo sapiens
K562, HepG2
characterized to standards
Status
released
Source (vendor)
Bethyl Labs
Product ID
A300-864A
Lot ID
2
Characterized targets
RBFOX2 (Homo sapiens)
Host
rabbit
Clonality
polyclonal
Purification
affinity
Isotype
IgG
Aliases
xiang-dong-fu:RBFOX2
External resources

Characterizations

RBFOX2 (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
Caption
IP-WB analysis of K562 whole cell lysate using RBFOX2 specific antibody. Lane 1 is 2.5% of 0.5mg input lysate, lane 2 is 2.5% of supernatant after immunoprecipitation and Lane 3 is 50% of IP enrichment using rabbit polyclonal anti-RBM9 Antibody. This antibody passes preliminary validation and will be further pursued for primary and secondary validation. Secondary validation is necessary becuase the predicted band in not recognized in the 'input/supernatant' sample due to low expression level.
Submitted by
Balaji Sundararaman
Lab
Gene Yeo, UCSD
RBFOX2 (Homo sapiens)
HepG2
Method: immunoprecipitation
Attachment from submitter
compliant
Caption
IP-Western Blot analysis of HepG2 whole cell lysate using IGF2BP1 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input, lane 2 is 1% of supernatant after IP and lane 3 is 10% of IP enrichment using rabbit polyclonal anti-RBFOX2 antibody (lanes under 'RBFOX2'). Lanes 4, 5 and 6 are in the same pattern but using rabbit normal IgG for IP (lanes under 'IgG').
Submitted by
Balaji Sundararaman
Lab
Gene Yeo, UCSD
RBFOX2 (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
compliant
Caption
IP-Western Blot analysis of K562 whole cell lysate using RBFOX2 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-RBFOX2 antibody (lanes under 'RBFOX2').
Submitted by
Steven Blue
Lab
Gene Yeo, UCSD
RBFOX2 (Homo sapiens)
Method: knockdown or knockout
Attachment from submitter
compliant
Caption
Western blot following shRNA against RBFOX2 in HepG2 whole cell lysate using RBFOX2 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against RBFOX2. RBFOX2 protein appears as the green band, Tubulin serves as a control and appears in red.
Submitted by
Xintao Wei
Lab
Brenton Graveley, UConn
RBFOX2 (Homo sapiens)
Method: knockdown or knockout
Attachment from submitter
compliant
Caption
Western blot following shRNA against RBFOX2 in K562 whole cell lysate using RBFOX2 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against RBFOX2. RBFOX2 protein appears as the green band, Tubulin serves as a control and appears in red.
Submitted by
Xintao Wei
Lab
Brenton Graveley, UConn