ENCAB592TEY
Antibody against Homo sapiens RBFOX2
Homo sapiens
HepG2, K562
characterized to standards
- Status
- released
- Source (vendor)
- Bethyl Labs
- Product ID
- A300-864A
- Lot ID
- 2
- Characterized targets
- RBFOX2 (Homo sapiens)
- Host
- rabbit
- Clonality
- polyclonal
- Purification
- affinity
- Isotype
- IgG
- Aliases
- xiang-dong-fu:RBFOX2
- External resources
Characterizations
RBFOX2 (Homo sapiens)
K562
Method: immunoprecipitation
not submitted for review by lab
- Caption
- IP-WB analysis of K562 whole cell lysate using RBFOX2 specific antibody. Lane 1 is 2.5% of 0.5mg input lysate, lane 2 is 2.5% of supernatant after immunoprecipitation and Lane 3 is 50% of IP enrichment using rabbit polyclonal anti-RBM9 Antibody. This antibody passes preliminary validation and will be further pursued for primary and secondary validation. Secondary validation is necessary becuase the predicted band in not recognized in the 'input/supernatant' sample due to low expression level.
- Submitted by
- Balaji Sundararaman
- Lab
- Gene Yeo, UCSD
- Grant
- U54HG007005
- Download
- Bethyl_A300-864A_2_RBFOX2.png
RBFOX2 (Homo sapiens)
HepG2
Method: immunoprecipitation
compliant
- Caption
- IP-Western Blot analysis of HepG2 whole cell lysate using IGF2BP1 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input, lane 2 is 1% of supernatant after IP and lane 3 is 10% of IP enrichment using rabbit polyclonal anti-RBFOX2 antibody (lanes under 'RBFOX2'). Lanes 4, 5 and 6 are in the same pattern but using rabbit normal IgG for IP (lanes under 'IgG').
- Submitted by
- Balaji Sundararaman
- Lab
- Gene Yeo, UCSD
- Grant
- U54HG007005
- Download
- HepG2_Bethyl_A300-864A_2_RBFOX2.png
RBFOX2 (Homo sapiens)
K562
Method: immunoprecipitation
compliant
- Caption
- IP-Western Blot analysis of K562 whole cell lysate using RBFOX2 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-RBFOX2 antibody (lanes under 'RBFOX2').
- Submitted by
- Steven Blue
- Lab
- Gene Yeo, UCSD
- Grant
- U54HG007005
- Download
- K562_Bethyl_A300-864A_2_RBFOX2.png
RBFOX2 (Homo sapiens)
Method: knockdown or knockout
compliant
- Caption
- Western blot following shRNA against RBFOX2 in HepG2 whole cell lysate using RBFOX2 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against RBFOX2. RBFOX2 protein appears as the green band, Tubulin serves as a control and appears in red.
- Submitted by
- Xintao Wei
- Lab
- Brenton Graveley, UConn
- Grant
- U54HG007005
- Download
- RBFOX2_H_Secondary_Western.png
RBFOX2 (Homo sapiens)
Method: knockdown or knockout
compliant
- Caption
- Western blot following shRNA against RBFOX2 in K562 whole cell lysate using RBFOX2 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against RBFOX2. RBFOX2 protein appears as the green band, Tubulin serves as a control and appears in red.
- Submitted by
- Xintao Wei
- Lab
- Brenton Graveley, UConn
- Grant
- U54HG007005
- Download
- RBFOX2_Secondary_Western.png