1. Antibodies
  2. Homo sapiens
  3. IKZF1

ENCAB590IRI

Antibody against Homo sapiens IKZF1

Homo sapiens
K562
characterized to standards
Homo sapiens
GM12878
characterized to standards with exemption
Homo sapiens
any cell type or tissue
partially characterized
Status
released
Source (vendor)
Bethyl Labs
Product ID
A303-516A
Lot ID
1
Characterized targets
IKZF1 (Homo sapiens)
Host
rabbit
Clonality
polyclonal
Purification
affinity
Aliases
michael-snyder:AS-1257
External resources

Characterizations

IKZF1 (Homo sapiens)
Method: motif enrichment
Attachment PDF Icon
compliant
Caption
The motif for target IKZF1 is represented by the attached position weight matrix (PWM) derived from ENCFF886VSU. Motif enrichment analysis was done by Dr. Zhizhuo Zhang (Broad Institute, Kellis Lab). Accept probability score: 0.672197682; Global enrichment Z-score: 2.040340156; Positional bias Z-score: 2.766127773; Peak rank bias Z-score: 1.151507911; Enrichment rank: 1.0.
Reviewer comment
The arrangement is above .65, so this qualifies
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
IKZF1 (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
exempt from standards
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: K562 using the antibody A303-516A. The image shows western blot analysis of input, flowthrough, immunoprecipitate, and mock immunoprecipitate using IgG. Target molecular weight: 57.528.
Submitter comment
IKZF1 has 8 isoforms, they are 57.5KD, 48KD, 43KD, 41KD, 32KD and 24KD. So we saw multiple bands.
Reviewer comment
Multiple bands and marked band is different between this IP and the previously submitted one in K562. However, IKZF1 is known to have multiple isoforms similar in size.
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
IKZF1 (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: K562, using the antibody A303-516A. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.
Submitted by
Denis Salins
Lab
Michael Snyder, Stanford
IKZF1 (Homo sapiens)
GM12878
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
exempt from standards
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: GM12878 using the antibody A303-516A. The image shows western blot analysis of input, flowthrough, immunoprecipitate, and mock immunoprecipitate using IgG. Target molecular weight: 57.528.
Submitter comment
IKZF1 has 8 isoforms, they are 57.5KD, 48KD, 43KD, 41KD, 32KD and 24KD. So we saw multiple bands.
Reviewer comment
Multiple bands and marked band is different between this IP and the previously submitted one in K562. However, IKZF1 is known to have multiple isoforms similar in size.
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
IKZF1 (Homo sapiens)
Method: immunoprecipitation followed by mass spectrometry
Attachment PDF Icon
not reviewed
Caption
IP followed by mass spectrometry. Briefly, protein was immunoprecipitated from K562 nuclear cell lysates using the antibody A303-516A, and the IP fraction was loaded on a 10% polyacrylamide gel (NuPAGEBis-Tris Gel) and separated with an Invitrogen NuPAGE electrophoresis system. The gel was stained by ColloidialCoomassie G-250 stain, gel fragments corresponding to the bands indicated were excised. Then proteins were trypsinized using the in-gel digestion method. Digested proteins were analyzed on an Orbitrap Elite mass spectrometer (Thermo Scientific) by the nanoLC-ESI-MS/MS technique. Peptides were identified by the SEQUEST algorithm and filtered with a high confidence threshold (Peptide false discovery rate < 1%, 2 unique peptides per protein minimum, mass error < 10 ppm).
Submitter comment
None of the proteins with 2 or more than 2 peptide counts have been shown to be sequence-specific TFs (including FUS, PTRF, HNRL1, ACL6A).
Reviewer comment
Not reviewed because this antibody has already been fully characterized in K562.
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
IKZF1 (Homo sapiens)
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
not reviewed
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line K562 using the antibody A303-516A. Lane 1: input nuclear lysate. Lane 2: material immunoprecipitated with antibody. Lane 3: material immunoprecipitated using control IgG. Marked bands were excised from gel and subjected to analysis by mass spectrometry. Target molecular weight: 57.528.
Reviewer comment
Not reviewed because this antibody has already been fully characterized in K562.
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford