ENCAB587QKH

Antibody against Homo sapiens PRDM10

Homo sapiens
K562
partially characterized
Status
released
Source (vendor)
Bethyl Labs
Product ID
A303-204A
Lot ID
1
Characterized targets
PRDM10 (Homo sapiens)
Host
rabbit
Clonality
polyclonal
Purification
affinity
Aliases
michael-snyder:AS-1587
External resources

Characterizations

PRDM10 (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
exempt from standards
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: K562, using the antibody A303-204A. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 130.136
Submitter comment
UniProt notes that PRDM10 has 7 isoforms, one is 137KD, one is 116KD.
Reviewer comment
Multiple bands and marked band not 50% of total signal in lane.
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
PRDM10 (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line K562 using the antibody A303-204A. Lane 1: input nuclear lysate. Lane 2: material immunoprecipitated with antibody. Lane 3: material immunoprecipitated using control IgG. Marked bands were excised from gel and subjected to analysis by mass spectrometry. Target molecular weight: 130.136.
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford