ENCAB582KNL
Antibody against Homo sapiens RING1
Homo sapiens
GM12878, SK-N-SH
partially characterized
Homo sapiens
K562, HepG2
not characterized to standards
- Status
- released
- Source (vendor)
- Abcam
- Product ID
- ab32644-100
- Lot ID
- 831561
- Characterized targets
- RING1 (Homo sapiens)
- Host
- rabbit
- Clonality
- polyclonal
- Antigen description
- PRC1 complex E3 ubiquitin ligase for lysine 119 of histone 2A
- Aliases
- bradley-bernstein:PchAb 260
- External resources
Characterizations
RING1 (Homo sapiens)
K562HepG2GM12878SK-N-SH
Method: immunoblot
compliant
- Caption
- Nuclear lysates from K562 (10 ug), HepG2 (7 ug), GM12878 (7 ug), SK-N-SH (6 ug) were loaded into a 4-12% Bis-Tris gel in 1X MOPS running buffer. After separation, the samples were transferred to a nitrocellulose membrane using iblot. Membrane was blocked for an hour in room temperature, with 5% BSA in TBS-T and blotted with primary antibody in the appropriate concentration over night at 4c. Membrane was washed and blotted with secondary HRP-conjugated antibody. Detection was made with Optiblot ECL Detect Kit (ab133406) for 3 seconds on BioRad ChemiDoc. Bands were detected at the expected size in HepG2, GM12878, and SK-N-SH cells, and a faint band at a lower molecular weight potentially associated with a known variant (http://www.uniprot.org/blast/?about=Q06587-2) was seen in K562 cells.
- Submitter comment
- Molecular mass: 42429 Da
- Submitted by
- Noam Shoresh
- Lab
- Bradley Bernstein, Broad
- Grant
- U54HG006991
- Download
- RING1_abcam_ab32644_831561_WB.png