ENCAB582KNL

Antibody against Homo sapiens RING1

Homo sapiens
SK-N-SH, GM12878
partially characterized
Homo sapiens
K562, HepG2
not characterized to standards
Status
released
Source (vendor)
Abcam
Product ID
ab32644-100
Lot ID
831561
Characterized targets
RING1 (Homo sapiens)
Host
rabbit
Clonality
polyclonal
Antigen description
PRC1 complex E3 ubiquitin ligase for lysine 119 of histone 2A
Aliases
bradley-bernstein:PchAb 260
External resources

Characterizations

RING1 (Homo sapiens)
K562HepG2GM12878SK-N-SH
Method: immunoblot
Attachment from submitter
compliant
Caption
Nuclear lysates from K562 (10 ug), HepG2 (7 ug), GM12878 (7 ug), SK-N-SH (6 ug) were loaded into a 4-12% Bis-Tris gel in 1X MOPS running buffer. After separation, the samples were transferred to a nitrocellulose membrane using iblot. Membrane was blocked for an hour in room temperature, with 5% BSA in TBS-T and blotted with primary antibody in the appropriate concentration over night at 4c. Membrane was washed and blotted with secondary HRP-conjugated antibody. Detection was made with Optiblot ECL Detect Kit (ab133406) for 3 seconds on BioRad ChemiDoc. Bands were detected at the expected size in HepG2, GM12878, and SK-N-SH cells, and a faint band at a lower molecular weight potentially associated with a known variant (http://www.uniprot.org/blast/?about=Q06587-2) was seen in K562 cells.
Submitter comment
Molecular mass: 42429 Da
Submitted by
Noam Shoresh
Lab
Bradley Bernstein, Broad